MEDICAL STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA

致病原虫的医学结构基因组学

• 批准号: 8362112
• 负责人: ETHAN A MERRITT
• 金额: $ 0.22万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362112
• 关键词: Active Sites; African Trypanosomiasis; Binding; Chagas Disease; Chemicals; Funding; Grant; L-Selenomethionine; Leishmania; Leishmaniasis; Medical; National Center for Research Resources; Phase; Plasmodium; Principal Investigator; Proteins; Protozoa; Radiation; Research; Research Infrastructure; Resources; Robotics; Screening procedure; Source; Structure; Trypanosoma; Trypanosoma cruzi; United States National Institutes of Health; cost; follow-up; pathogen; small molecule; structural biology; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This proposal encompasses the initial structure determination of medically relevant target proteins from the eukaryotic pathogens Trypanosoma bruci (African sleeping sickness). T. cruzi (Chagas disease), Leishmania spp. (leishmaniasis) and Plasmodium spp. (maleria), and follow-up screening of small molecule cocktails to identify chemical fragments that bind at the active site. Initial structure determinations will rely primarily on SeMet MAD/SAD phasing. Screening of cocktail soaks can be done at fixed wavelength, but benifits greatly from the use of robotic automounting and screening.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该提案涵盖了来自真核病原体锥虫瘤（非洲睡眠疾病）的医学相关靶蛋白的初始结构测定。 T. Cruzi（Chagas病），Leishmania spp。 （利什曼病）和疟原虫。 （Maleria），以及对小分子鸡尾酒的后续筛选，以鉴定在活性位点结合的化学片段。初始结构确定将主要依赖于SEMET MAD/SAD相位。 可以在固定的波长下进行鸡尾酒浸泡的筛选，但由于使用机器人自动启动和筛选而大大促进了鸡尾酒浸泡。