MEDICAL STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 该建议包括从真核病原体布氏锥虫（非洲昏睡病）的医学相关的靶蛋白的初始结构测定。T. cruzi（恰加斯病）、利什曼原虫属（Leishmania spp.）（利什曼病）和疟原虫属（Plasmodium spp.）（maleria），并对小分子混合物进行后续筛选以鉴定在活性位点结合的化学片段。初始结构测定将主要依赖于SeMet MAD/SAD定相。 鸡尾酒浸泡液的筛选可以在固定波长下进行，但使用机器人自动装载和筛选会大大受益。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal encompasses the initial structure determination of medically relevant target proteins from the eukaryotic pathogens Trypanosoma bruci (African sleeping sickness). T. cruzi (Chagas disease), Leishmania spp. (leishmaniasis) and Plasmodium spp. (maleria), and follow-up screening of small molecule cocktails to identify chemical fragments that bind at the active site. Initial structure determinations will rely primarily on SeMet MAD/SAD phasing. Screening of cocktail soaks can be done at fixed wavelength, but benifits greatly from the use of robotic automounting and screening.