MEDICAL STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项建议包括确定来自真核病原体布鲁氏锥虫(非洲昏睡病)的具有医学意义的目标蛋白的初始结构。克鲁兹毛滴虫(恰加斯病)、利什曼原虫(Leishmania spp.(利什曼病)和疟原虫。(马来病)，以及小分子鸡尾酒的后续筛选，以确定结合在活性部位的化学碎片。初始结构的确定将主要取决于SEMET MAD/SAD阶段。鸡尾酒浸泡的筛选可以在固定波长下进行，但机器人自动安装和筛选的使用带来了极大的好处。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal encompasses the initial structure determination of medically relevant target proteins from the eukaryotic pathogens Trypanosoma bruci (African sleeping sickness). T. cruzi (Chagas disease), Leishmania spp. (leishmaniasis) and Plasmodium spp. (maleria), and follow-up screening of small molecule cocktails to identify chemical fragments that bind at the active site. Initial structure determinations will rely primarily on SeMet MAD/SAD phasing. Screening of cocktail soaks can be done at fixed wavelength, but benifits greatly from the use of robotic automounting and screening.