PROTEIN QUANTIFICATION FOR MOUSE MODEL OF BREAST CANCER

乳腺癌小鼠模型的蛋白质定量

• 批准号: 8361420
• 负责人: RON BOSE
• 金额: $ 1.42万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361420
• 关键词: Data Analyses; Experimental Designs; Funding; Grant; Label; Mass Spectrum Analysis; Modeling; National Center for Research Resources; Phase; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Resources; Source; Transgenic Mice; United States National Institutes of Health; biomedical resource; cost; malignant breast neoplasm; mouse model; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is a two phase project with the first being the optimization of label-free protein quantification for detecting differentially expressed proteins in a transgenic mouse model of breast cancer. The second phase is to use the quantification model to discover novel breast cancer effected proteins. Both phases will use the standard LC-MS/MS platform in use with the proteomics core, but will use statistical experimental design and a novel application of linear mixed models for data analysis.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这是一个两个阶段的项目，第一个阶段是优化无标记蛋白质 转基因小鼠差异表达蛋白的定量检测 乳腺癌的模型。第二阶段是使用量化模型来发现 新的乳腺癌影响蛋白。两个阶段都将使用标准LC-MS/MS 平台使用蛋白质组学核心，但将使用统计实验设计和 线性混合模型在数据分析中的新应用。