IMPACT OF VIRAL-MEDIATED IGF-I GENE TRANSFER ON SKELETAL MUSCLE FOLLOWING

病毒介导的 IGF-I 基因转移对骨骼肌的影响

• 批准号: 8361458
• 负责人: KRISTA H VANDENBORNE
• 金额: $ 1.22万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361458
• 关键词: Age; Anterior; Binding Proteins; Female; Funding; Gene Transfer; Grant; Hindlimb; Immobilization; Insulin-Like Growth Factor I; Insulin-Like-Growth Factor I Receptor; Mass Spectrum Analysis; Mediating; Molecular; Mus; Muscle; National Center for Research Resources; Play; Positioning Attribute; Principal Investigator; Protein Binding; Recombinant adeno-associated virus (rAAV); Recovery; Research; Research Infrastructure; Resources; Role; Skeletal Muscle; Source; United States National Institutes of Health; Viral; adeno-associated viral vector; biomedical resource; cost; muscle hypertrophy; muscle regeneration; overexpression; receptor; response; tibialis anterior muscle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Insulin-like growth factor I (IGF-I) is a potent myogenic factor that plays a critical role in muscle regeneration and muscle hypertrophy. The purpose of this study was to evaluate the effect of IGF-I overexpression on the recovery of muscle size and function during reloading/reambulation after a period of cast immobilization in predominantly fast twitch muscles. In addition, we investigated concomitant molecular responses in IGF-I receptor and binding proteins (BPs). Recombinant adeno-associated virus vector for IGF-I (rAAV-IGF-IA) was injected into the anterior compartment of one of the hindlimbs of young (3 wk) C57BL6 female mice. At 20 wk of age, both hindlimbs were cast immobilized in a shortened position for 2 wk to unload the tibialis anterior (TA) and extensor longus digitorum (EDL) muscles. The TA and EDL muscles were removed bilaterally after 2 wk of cast immobilization and after 1 and 3 wk of free cage reambulation.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 胰岛素样生长因子I（IGF-I）是一种有效的肌源性因子，在肌肉再生和肌肉肥大中起关键作用。本研究的目的是评估胰岛素样生长因子-I过表达对恢复肌肉大小和功能的影响，在重装/reamestation后一段时间的石膏固定在主要是快收缩肌肉。此外，我们研究了伴随的IGF-I受体和结合蛋白（BP）的分子反应。将IGF-I的重组腺相关病毒载体（rAAV-IGF-IA）注射到年轻（3周）C57 BL 6雌性小鼠的一条后肢的前室中。在20周龄时，将两条后肢以缩短的位置固定2周，以卸载胫骨前肌（TA）和趾长伸肌（EDL）。在石膏固定2周后以及游离笼重建1周和3周后，切除双侧TA和EDL肌肉。