PROTEOMIC ANALYSIS OF FOLLICULAR FLUID AND SERA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 莫利实验室正在进行无标记蛋白质组学研究，旨在 确定体外受精(IVF)成功的预测因素。补语 级联是动物早期妊娠发育的关键，但其作用 补体蛋白在体外受精成功中的作用尚不清楚。我们推测补充物 卵泡微环境中的活动影响卵母细胞的能力和体外受精的结果。 血清和卵泡液样本将从正在接受治疗的患者身上分析 试管受精的目的是确定成功试管受精的潜在生物标志物。 蛋白质组学分析揭示了卵泡液中补体蛋白的特征 实现怀孕的患者。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Moley lab is undertaking label free proteomics studies that are geared toward determining predictive factors for successful in vitro fertilization (IVF). The complement cascade is critical for early pregnancy development in animals, but the role of complement proteins in success of IVF is unknown. We speculate that complement activity in the follicular microenvironment affects oocyte competence and IVF outcomes. Serum and follicular fluid samples will be analyzed from patients who are undergoing IVF for the purpose of identifying potential biomarkers for successful IVF outcome. Proteomic analysis reveals a signature of complement proteins in the follicular fluid of patients achieving pregnancy.