PROTEOMIC ANALYSIS OF FOLLICULAR FLUID AND SERA

卵泡液和血清的蛋白质组分析

• 批准号: 8361414
• 负责人: KELLE H MOLEY
• 金额: $ 1.01万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361414
• 关键词: Affect; Animals; Biological Markers; Competence; Complement; Development; Fertilization in Vitro; Follicular Fluid; Funding; Grant; Label; Mass Spectrum Analysis; National Center for Research Resources; Oocytes; Outcome; Patients; Predictive Factor; Pregnancy; Principal Investigator; Proteomics; Research; Research Infrastructure; Resources; Role; Sampling; Serum; Source; United States National Institutes of Health; biomedical resource; cost; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Moley lab is undertaking label free proteomics studies that are geared toward determining predictive factors for successful in vitro fertilization (IVF). The complement cascade is critical for early pregnancy development in animals, but the role of complement proteins in success of IVF is unknown. We speculate that complement activity in the follicular microenvironment affects oocyte competence and IVF outcomes. Serum and follicular fluid samples will be analyzed from patients who are undergoing IVF for the purpose of identifying potential biomarkers for successful IVF outcome. Proteomic analysis reveals a signature of complement proteins in the follicular fluid of patients achieving pregnancy.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 莫利实验室正在进行无标签蛋白质组学研究， 确定成功体外受精（IVF）的预测因素。补体 级联反应对于动物的早期妊娠发育至关重要，但 补体蛋白在IVF成功中的作用尚不清楚。我们推测补体 卵泡微环境中的活性影响卵母细胞的能力和IVF结果。 将分析来自正在接受化疗的患者的血清和卵泡液样本。 IVF旨在确定成功IVF结局的潜在生物标志物。 蛋白质组学分析揭示了卵泡液中补体蛋白的特征， 患者怀孕。