MECHANISMS OF X-RAY RADIATION DAMAGE TO PROTEIN CRYSTALS

X 射线辐射损伤蛋白质晶体的机制

• 批准号: 8363568
• 负责人: Robert E. Thorne
• 金额: $ 1.02万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363568
• 关键词: Electrons; Funding; Grant; Insulin; National Center for Research Resources; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Research Personnel; Resources; Roentgen Rays; Source; Structure; Temperature; United States National Institutes of Health; X-Ray Crystallography; Zinc; cost; cryogenics; design; protein degradation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Degradation of protein crystals by X-ray beams is an important factor in the design of an X-ray crystallography experiment. Despite the fact that researchers have been using cryogenic temperatures to mitigate radiation damage for over 20 years, the underlying mechanisms involved are still the subject of debate. In this round of experiments, we intend to isolate the contribution to radiation damage that comes from the presence of a heavy atom. By looking at the way radiation damage is manifested in the structure just above and below the zinc edge in zinc-containing insulin crystals, we will be able to determine the effect of e.g. Auger electrons from the zinc core-hole.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 X 射线束对蛋白质晶体的降解是 X 射线晶体学实验设计中的一个重要因素。 尽管研究人员20多年来一直在使用低温来减轻辐射损伤，但所涉及的潜在机制仍然是争论的主题。 在这一轮的实验中，我们打算隔离重原子的存在对辐射损伤的影响。 通过观察含锌胰岛素晶体中锌边缘上方和下方结构中辐射损伤的表现方式，我们将能够确定例如辐射损伤的影响。来自锌核孔的俄歇电子。