FIBER DIFFRACTION FROM CELLULOSE IN GENETICALLY MODIFIED PLANTS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 生物学中一个有趣的问题是，植物如何在分子水平上控制生长过程中细胞壁的结构。我们建议探测转基因植物中的纤维素结构，在植物部分，如茎或根，生长速度显着快于野生型。在最后一次运行中，我们在D线上获得了广角散射/纤维衍射的第一个结果，然而，数据受到背景水平和有限的检测器面积和分辨率的限制。在F1，我们希望使用为蛋白质微晶体学工作开发的氦气环境，结合100微米准直器或聚焦毛细管。 本开发研究的目的是了解哪些特性对于本实验的成功至关重要，我们希望获得尽可能详细的纤维素结构数据（氦气、室温下的预湿氦气、低温下的干氦气、检测器范围、信噪比）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A fascinating problem in biology is, how plants control the structure of their cell walls during growth on a molecular level. We propose to probe the cellulose structure in genetically modified plants, in plant parts such as stems or roots which grow significantly faster than in the wildtype. We obtained first results with wide-angle scattering/fiber diffraction on D-line in the last run, however, the data were limited by background levels and the limited detector area and resolution. At F1 we would like to use the helium environment developed for the protein microcrystallography effort, in combination with either a 100 micron collimator or a focusing capillary. Goal of this development study is to see, which features are essential to the success of this experiment where we would like to obtain as detailed data on the cellulose structure as possible (helium, prewetted helium at room temperature, dry helium for cryogenic temperature, detector range, signal-to-noise).