THE APPLICATION OF SOLUTION SCATTERING TO THE STUDY OF VIRUS-HOST INTERACTIONS

溶液散射在病毒-宿主相互作用研究中的应用

• 批准号: 8363553
• 负责人: WAYNE L SCHULTZ
• 金额: $ 0.1万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363553
• 关键词: Binding; Cell Nucleus; Complex; Funding; Grant; Influenza; Karyopherins; Mediating; N-terminal; National Center for Research Resources; Nuclear Localization Signal; Pathway interactions; Peptide Signal Sequences; Principal Investigator; Proteins; Reporting; Research; Research Infrastructure; Resources; Solutions; Source; United States National Institutes of Health; Viral Proteins; Virus; cost; insight; interest; karyopherin alpha 2; nucleocytoplasmic transport; protein transport; tool; virus host interaction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Karyopherin alpha 2 (KPNA2) facilitates protein transport into the nucleus through recognition of a nuclear localization signal (NLS). Many viruses utilize the karyopherins of the nuclear transport pathway to transport viral proteins into the nucleus. Of interest, an NLS sequence (RKLKR) has been reported in the N-terminal region of the Influenza A Matrix 1 (InfAM1) protein, and presents itself as a putative binding partner to KPNA2. The mechanism by which InfAM1 is shuttled into the nucleus is likely to be similar to the transport of host endogenous proteins that is mediated by the karyopherins. We wish to use SAXS as a complementary tool to determine if said interaction exist, and a gain insight as to how the complex looks.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 核转运蛋白α 2（KPNA 2）通过识别核定位信号（NLS）促进蛋白质转运到细胞核中。许多病毒利用核转运途径的核转运蛋白将病毒蛋白质转运到细胞核中。感兴趣的是，NLS序列（RKLKR）已在甲型流感病毒基质1（InfAM 1）蛋白的N-末端区域中报道，并将其本身作为KPNA 2的推定结合伴侣。 InfAM 1穿梭进入细胞核的机制可能与由核转运蛋白介导的宿主内源性蛋白质的转运相似。我们希望使用SAXS作为一种补充工具来确定是否存在所述相互作用，并深入了解复杂的外观。