LAUE DIFFRACTION FROM MICROCRYSTALS WITH A NARROW-BANDPASS BEAM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 之前在第一太平戴维斯的实验已经证明，使用宽带通光束，在低温和室温下，从大小约30微米的蛋白质晶体收集劳埃数据是可行的。我们要求在G1处的时间：(1)比较宽(25%)和窄(1.5%)带通的数据质量，(2)查看是否可以使用G1处的强烈摆动光束从尺寸为&lt；10微米的晶体中获得有用的数据，(3)尝试从含有锌的蛋白质中获得异常数据，用于SAD相位。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Previous experiments at D1 have demonstrated the feasibility of collecting Laue data from protein crystals down to about 30 microns in size, at both cryo and room temperature, using a wide-bandpass beam. We request time at G1 to: (1) compare data quality with wide (25%) and narrow (1.5%) bandpasses, (2) see whether useful data can be obtained from crystals of < 10 microns size using the intense wiggler beam at G1, (3) attempt to obtain anomalous data from proteins containing zinc, for SAD phasing.