PIF3 PHOSPHORYLATION SITES AND ASSOCIATED PROTEINS

PIF3 磷酸化位点和相关蛋白

• 批准号: 8363817
• 负责人: Peter H. Quail
• 金额: $ 1.89万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363817
• 关键词: Address; Cell Nucleus; Cytoplasm; Data; Development; Environment; Exposure to; Family; Funding; Gene Targeting; Grant; Light; Mass Spectrum Analysis; National Center for Research Resources; Phosphorylation; Phosphorylation Site; Photoreceptors; Phytochrome; Plants; Play; Principal Investigator; Process; Protein Kinase; Proteins; Repression; Research; Research Infrastructure; Resources; Role; Sensory; Signal Transduction; Source; System; Ubiquitin; United States National Institutes of Health; computerized data processing; cost; member; multicatalytic endopeptidase complex; response; transcription factor; ubiquitin-protein ligase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The phytochrome (phy) family of sensory photoreceptors control plant adaptational responses to light signals from the environment. Current data indicate that the signal transduction process involves rapid translocation of the light-activated photoreceptor molecule from the cytoplasm to the nucleus, where it interacts physically with a subset of members of the bHLH transcription-factor family, termed phytochrome-interacting factors (PIFs), inducing transcriptional responses in target genes. Recent evidence shows that members of the PIF family repress development in the dark, and that photoactivated phy reverses this repression by inducing rapid degradation of the PIF molecules upon exposure to light. This process involves rapid, phy-induced phosphorylation of the interacting bHLH protein, followed by degradation via the ubiquitin proteasome system. The specific, primary focus of this segment of the project is to identify the light-induced phosphorylation sites in the PIF3 molecule, as a step toward understanding the mechanism of phosphorylation and the role it plays in tagging the bHLH factor for ubiquitylation. A second related objective is to identify proteins that are induced by light to interact with the PIF3 protein as a step toward identifying the protein kinase and E3 ligase responsible for the phosphorylation and ubiquitylation steps in the signaling process. It is proposed to use mass spec analysis to address both these objectives.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 光敏色素(PHY)家族感光感受器控制植物对环境光信号的适应性反应。目前的数据表明，信号转导过程涉及光激活的光感受器分子从细胞质快速移位到核，在那里它与bHLH转录因子家族的一部分成员物理上相互作用，称为光敏色素相互作用因子(PIFs)，在靶基因中诱导转录反应。最近的证据表明，PIF家族的成员在黑暗中抑制发育，而光激活PHY通过诱导PIF分子在光照下快速降解来逆转这种抑制。这个过程包括PHY诱导相互作用的bHLH蛋白的快速磷酸化，然后通过泛素蛋白酶体系统降解。该项目的这一部分的主要重点是确定光诱导的PIF3分子中的磷酸化位点，作为了解磷酸化机制及其在标记bHLH因子进行泛素化方面所起的作用的一步。第二个相关的目标是鉴定受光诱导与PIF3蛋白相互作用的蛋白质，作为鉴定信号过程中负责磷酸化和泛素化步骤的蛋白激酶和E3连接酶的一步。建议使用质谱分析来解决这两个目标。