USING HIGH-RESOLUTION CRYSTAL STRUCTURES TO GUIDE THE ENGINEERING OF REAGENT ENZ

使用高分辨率晶体结构指导试剂 ENZ 的工程设计

• 批准号: 8362413
• 负责人: MOLLY MIN HE
• 金额: $ 0.06万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362413
• 关键词: Active Sites; Biological Sciences; Chemistry; Complex; DNA; DNA-Directed DNA Polymerase; Engineering; Enzymes; Exonuclease; Funding; Grant; Ligase; National Center for Research Resources; Nucleic Acids; Nucleotides; Play; Polymerase; Principal Investigator; RNA-Directed DNA Polymerase; Radiation; Reagent; Research; Research Infrastructure; Resolution; Resources; Role; Source; Structure; United States National Institutes of Health; cost; design; novel; restriction enzyme; structural biology; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Reagent enzymes are the unsung heroes in the field of life sciences research. From restriction enzymes, ligases, to polymerases and exonucleaes, these enzymes play critical roles in all types of nucleic acid analysis. Here we propose to use SSRL x-ray source to obtain high-resolution crystal structures of various reagent enzymes including DNA polymerase, exonuclease, reverse transcriptase, and their complexes with DNA and proprietary nucleotides. The structural information obtained will be utilized to design novel active site chemistries of these enzymes as better tools for life sciences research.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 试剂酶是生命科学研究领域的无名英雄。 从限制酶、连接酶到聚合酶和外切核酸酶，这些酶在所有类型的核酸分析中起着关键作用。 在这里，我们建议使用SSRL X射线源，以获得高分辨率的晶体结构的各种试剂酶，包括DNA聚合酶，核酸外切酶，逆转录酶，以及它们的复合物与DNA和专有的核苷酸。 所获得的结构信息将用于设计这些酶的新活性位点化学，作为生命科学研究的更好工具。