FLEXIBILITY OF THE HIV TAR RNA STUDIED USING SMALL-ANGLE X-RAY SCATTERING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目将使用小角X射线散射(SAXS)测量艾滋病毒反式激活反应元件(TAR)的折叠。TAR形成螺旋-连接-螺旋，这是RNA中的一个基本结构基序，也是RNA折叠的基础，从而提供了对大多数结构RNA的洞察。TAR在医学上也与其在艾滋病毒生命周期中的作用有关。这个项目将探索在氨基糖苷类和反离子存在的情况下HIV焦油的结构，并将其与现有模型和核磁共振数据进行比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project will measure the folding of the transactivation response element (TAR) from HIV using small-angle X-ray scattering (SAXS). TAR forms a helix-junction-helix, a basic structural motif in RNA and the basis of RNA folding, thus providing insight into most structured RNAs. TAR is also medically relevant for its role in the lifecycle of HIV. This project will probe the structure of HIV TAR in the presence of aminoglycosides and counterions, and compare it to existing models and NMR data.