FLEXIBILITY OF THE HIV TAR RNA STUDIED USING SMALL-ANGLE X-RAY SCATTERING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目将使用小角X射线散射（SAXS）测量HIV反式激活反应元件（TAR）的折叠。 TAR形成螺旋-连接-螺旋，这是RNA中的基本结构基序，也是RNA折叠的基础，因此提供了对大多数结构化RNA的深入了解。 TAR在HIV生命周期中的作用也具有医学相关性。 该项目将在氨基糖苷类和抗衡离子的存在下探测HIV TAR的结构，并将其与现有模型和NMR数据进行比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project will measure the folding of the transactivation response element (TAR) from HIV using small-angle X-ray scattering (SAXS). TAR forms a helix-junction-helix, a basic structural motif in RNA and the basis of RNA folding, thus providing insight into most structured RNAs. TAR is also medically relevant for its role in the lifecycle of HIV. This project will probe the structure of HIV TAR in the presence of aminoglycosides and counterions, and compare it to existing models and NMR data.