CRYSTAL STRUCTURE OF AMINOACYL-TRNA SYNTHETASES AT MULTI-FUNCTIONAL STATES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 氨基酰基tRNA合成酶催化氨基酸与其同源tRNA的结合。他们在蛋白质合成的第一反应中建立了遗传密码的算法。令人惊讶的是，细胞可以驱动这些家务机械在关键的信号转导途径中发挥新的功能，而不是翻译。例如，当肥大细胞免疫激活时，哺乳动物赖氨酰-tRNA合成酶(LysRS)被磷酸化并移位到细胞核。它与转录因子MITF结合，激活MITF依赖的基因表达。这些新的功能需要构象开关将合成酶从翻译重定向到信号转导。这些变化的细节尚不清楚。在这里，我们的目标是收集SSRL上不同状态的人类AARS的晶体衍射数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Aminoacyl tRNA synthetases catalyze the attachment of amino acids to their cognate tRNAs. They establish the algorithm of the genetic code in this first reaction of protein synthesis. Surprisingly, cells can drive these housekeeping machineries into novel functions in key signal transduction pathways beyond translation. For example, upon immune activation in mast cells, mammalian lysyl-tRNA synthetase (LysRS) is phosphorylated and translocated to the nucleus. It binds to transcription factor MITF, leading to the activation of MITF-dependent gene expression. These novel functions require conformational switch to redirect the synthetases from translation to signal transduction. Not known are the particulars of such changes. Here we aim to collect crystal diffraction data of the human aaRSs at different states at SSRL.