LIPOPOLYSACCHARIDE TRANSPORT

脂多糖运输

• 批准号: 8363366
• 负责人: DAVID H SHERMAN
• 金额: $ 0.42万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363366
• 关键词: Antibiotics; Biochemical; Crystallography; Development; Family; Funding; Gram-Negative Bacteria; Grant; Light; Lipopolysaccharides; Membrane; Membrane Proteins; National Center for Research Resources; Permeability; Phospholipids; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; Synchrotrons; United States National Institutes of Health; chemical genetics; cost; membrane assembly; periplasm; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The outer membrane of Gram-Negative Bacteria is an important permeability barrier that consists of an asymmetric bilayer in which the inner leaflet is primarily composed of phospholipids and the outer leaflet is primarily composed of lipopolysaccharides (LPS). Outer membrane proteins (OMPs) of the ?-barrel family span the bilayer and serve as channels and transporters. Using a chemical genetic approach, we recently identified a multi-protein complex that spans the periplasm that is responsible for the assembly of LPS. We are currently pursuing biochemical and structural studies of these proteins in order to understand how the hydrophobic LPS molecule is transported to and assembled in the outer leaflet of the OM. We hope to identify whether there are general principles that guide the assembly of this membrane and to characterize these new protein targets for antibiotic development.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 革兰氏阴性细菌的外膜是一个重要的渗透性屏障，由不对称双层组成，其中内部小叶主要由磷脂组成，外部小叶主要由脂多糖（LPS）组成。 ？ - 桶系列的外膜蛋白（OMP）跨越双层，并用作通道和转运蛋白。使用化学遗传学方法，我们最近确定了一种多蛋白质复合物，该复合物涵盖了负责LPS组装的周期。我们目前正在对这些蛋白质进行生化和结构研究，以了解疏水性LPS分子如何运输到OM的外部小叶中并组装。我们希望确定是否有一些一般原则可以指导该膜的组装，并表征这些新的蛋白质靶标的抗生素发育。