LYSOZYME + METAL ATOM

溶菌酶金属原子

• 批准号: 8363401
• 负责人: Yi Lu
• 金额: $ 0.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363401
• 关键词: Area; Catalysis; Crystallography; Devices; Funding; Grant; Growth; Hybrids; Investigation; Ions; Kinetics; Light; Metal Binding Site; Metals; Molecular; Muramidase; National Center for Research Resources; Optics; Play; Positioning Attribute; Principal Investigator; Reaction; Research; Research Infrastructure; Resources; Role; Source; Surface; Synchrotrons; Techniques; Transmission Electron Microscopy; United States National Institutes of Health; X-Ray Crystallography; biomineralization; cost; nano; nanomaterials; nanoparticle; nanoscale; nanoscience; plasmonics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Nano-scaled materials play a very important role in both scientific and applied areas. Though much progress has been made in nano-science, the investigation of nanomaterials' growth is still great challenge. In this project, we are using single crystals of lysozyme to slow down the growth of metal nanoparticles and thus study their growth kinetics at the molecular level. Lysozyme was chosen for this project because it not only can be readily crystallized, but also has a unique surface-exposed His at position 15 as a specific binding site for metal ions. Our primary results showed that lysozyme crystals could slow the reaction and thus we could get the snapshots of the interactions between lysozyme and the metal ions by X-ray crystallography. With the help of other techniques (such as transmission electron microscopy), we will elucidate the reaction mechanism of metal nanoparticles' growth within the lysozyme crystals, which might have implications in fabrication of bio-nano hybrid materials, biomineralization, catalysis, optical and plasmonic devices, and sensing.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 纳米材料在科学和应用领域都发挥着非常重要的作用。尽管纳米科学已经取得了很大进展，但纳米材料生长的研究仍然是巨大的挑战。在这个项目中，我们使用溶菌酶单晶来减缓金属纳米颗粒的生长，从而在分子水平上研究它们的生长动力学。该项目选择溶菌酶是因为它不仅易于结晶，而且在15位具有独特的表面暴露His作为金属离子的特异性结合位点。我们的初步结果表明，溶菌酶晶体可以减缓反应，因此我们可以通过 X 射线晶体学获得溶菌酶与金属离子之间相互作用的快照。借助其他技术（如透射电子显微镜），我们将阐明金属纳米粒子在溶菌酶晶体内生长的反应机制，这可能对生物纳米杂化材料的制造、生物矿化、催化、光学和等离子体装置以及传感产生影响。