BIOMASS TRANSFORMATION ? CATALYSIS OF ISPG

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 IspG是一种41kD的蛋白质，在生物燃料生产的宿主生物工程生产中扮演着关键的催化剂角色。根据刘和他的同事的研究结果，IspG以多蛋白复合体的形式存在。由于IspG催化还原脱氧反应，它们的伴侣蛋白要么参与电子传递，要么与IspG还原电位的调节有关。为了更好地了解这些蛋白质的作用机制，提高IspG的活性，我们对这些蛋白质进行了质谱鉴定。对这些伙伴中的每一个进行凝胶内消化，然后用MALDI-TOF MS和NanoLC-MS/MS对LTQ-Orbitrap(Thermo-Fisher)进行分析，以确定这些伙伴。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. IspG is a protein of 41 kD, known to play a key role as a catalyst in the engineering production of host organisms for biofuel production. According to the research results of Liu and his coworkers, IspG is present as mutli-protein-complexes. Because IspG catalyses reductive deoxygenation reactions, their partner proteins are either involved in electron transfer or related to the regulation of IspG reduction potential. To have a better understanding of the mechanism and improve the IspG activity, we have undeertaken the identification of these proteins by mass spectrometry. In-gel digestion of each of these partners followed by analysis by MALDI-TOF MS and nanoLC-MS/MS on LTQ-Orbitrap (Thermo-Fisher) are being performed to identify these partners.