TRAINING IN FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY

傅里叶变换离子回旋共振质谱培训

• 批准号: 8365521
• 负责人: CHI-WEI LIN
• 金额: $ 0.77万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365521
• 关键词: Biochemistry; Biology; Boston; Chemistry; Complex; Custom; Dissociation; Doctor of Philosophy; Electrons; England; Fourier Transform; Fourier transform ion cyclotron resonance; Funding; Germany; Grant; Infusion procedures; Ions; Korea; Laboratory Personnel; Left; Mass Spectrum Analysis; Medicine; Methods; National Center for Research Resources; Performance; Positioning Attribute; Postdoctoral Fellow; Principal Investigator; Proteins; Reporting; Research; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Resources; SHFM1 gene; Sampling; Science; Scientist; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Students; Techniques; Training; United States National Institutes of Health; Universities; Update; Work; base; cost; deamidation; falls; graduate student; instrument; mass spectrometer; medical schools; professor; programs; research study; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. One of the objectives of this program is to train new graduate students and professional scientific researchers in new techniques. One such technique which we have focused on is Fourier transform mass spectrometry. Since the departure of Dr. Peter O'Connor in Jan 2009 to become a tenured full professor in the Department of Chemistry at the University of Warwick, Coventry, England, Dr. Cheng Lin has assumed responsibility for training in FTMS, and FTMS-based research projects. He is now a research assistant professor in the Department of Biochemistry at Boston University School of Medicine, and the Assistant Director of the Resource. Updates for the reporting period of April 2010 - March 2011: A graduate student from the FTMS research group, Xiaojuan Li, defended her Ph. D. thesis, "Mechanism of electron capture dissociation and the application to protein deamidation", in July 2010, and subsequently joined Prof. Anderson's group as a postdoctoral research associate at Yale University. A former postdoc, Dr. Konstantin Aizikov left in December 2010 to take a research scientist position at Thermo Fisher Scientific at Bremen in Germany. In December 2010, Dr. Sang Yun Han from Korea Research Institute of Standards and Science joined the FTMS group to begin his 9-month sabbatical. He will be working primarily on developing tandem mass spectrometry methods for MALDI-generated ions using the custom-built MALDI-FTICR-MS instrument. In fall 2010, the IR option has been successfully set up on the 12 T Bruker solariX FT-ICR instrument, which enabled performance of a wide variety of experiments, including activated ion (AI)-ECD, post-ETD complex activation, and IRMPD. An Advion Nanomate has also been recently set up to allow automated infusion of sample into the mass spectrometer and a more efficient and stable spray. A number of graudate students and research scientists have been properly trained to operate this high-performance instrument, either independently or under the guidance of senior laboratory personnel.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该计划的目标之一是培养新的研究生和新技术的专业科学研究人员。 我们所关注的一种这样的技术是傅立叶变换质谱法。 自2009年1月Peter奥康纳博士离职成为英国考文垂沃里克大学化学系终身教授以来，Cheng Lin博士负责FTMS培训和基于FTMS的研究项目。他现在是波士顿大学医学院生物化学系的研究助理教授，也是资源部的助理主任。 2010年4月至2011年3月报告期的最新情况： FTMS研究小组的研究生李晓娟（音译）为自己的博士学位进行了答辩。于2010年7月发表题为“电子捕获解离机制及其在蛋白质脱酰胺中的应用”的论文，随后加入安德森教授的团队，在耶鲁大学担任博士后研究助理。 Konstantin Aizikov博士曾是一名博士后，于2010年12月离开，在德国不莱梅的赛默飞世尔科技公司担任研究科学家一职。 2010年12月，韩国标准科学研究院的Sang Yun Han博士加入FTMS小组，开始为期9个月的休假。他将主要致力于使用定制的MALDI-FTICR-MS仪器开发MALDI产生的离子的串联质谱方法。 2010年秋季，在12 T Bruker solariX FT-ICR仪器上成功设置了IR选项，可进行各种实验，包括活化离子（AI）-ECD、ETD后络合物活化和IRMPD。最近还建立了一个Advion Nanomate，可以将样品自动注入质谱仪，并实现更有效和稳定的喷雾。一些研究生和研究科学家已经接受了适当的培训，以独立或在高级实验室人员的指导下操作这种高性能仪器。