SPHERICAL VIRUS STRUCTURES

球形病毒结构

• 批准号: 8362444
• 负责人: THOMAS SMITH
• 金额: $ 0.64万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362444
• 关键词: Address; Adult; Affect; Animal Model; Animals; Antibodies; Back; Basic Science; Calicivirus; Capsid; Cell Culture System; Economics; Epidemic; Funding; Gastroenteritis; Genetic; Grant; Health; Human; Immunity; Immunologics; Immunology; Laboratories; Microscopy; Molecular; Morbidity - disease rate; Mus; National Center for Research Resources; Norovirus; Pathogenesis; Principal Investigator; Research; Research Infrastructure; Resources; Source; Species Specificity; Structure; United States National Institutes of Health; Vaccinated; Vaccination; Virus; attenuation; cost; receptor binding; tool; virology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. MNV is the first murine calcivirus structure and neutralization studies. Human caliciviruses are the major cause of epidemic gastroenteritis in adults, and thereby cause significant morbidity and economic loss. Important to human health, it is not known whether it is possible to effectively vaccinate against these agents, and mechanisms of vaccination are undefined. Thus, the lack of a small animal model with extensive genetic and immunologic tools for the analysis of pathogenesis and immunity has held back research on these important viruses. The H.W. Virgin laboratory has discovered the first murine calicivirus that they have designated Mouse Norovirus 1 or MNV-1. Unique amongst the Caliciviruses, they have developed both a whole animal and cell culture system. This affords new opportunities for basic research into calicivirus virology and immunology. Specifically, some of the questions we can address by analyzing the various structures of MNV-1: ¿ What are the important structural determinants that are recognized by antibodies that confer protection? ¿ What types of structural changes result in attenuation of the virus? ¿ Is the capsid dynamic and do neutralizing/protecting antibodies act by affecting these dynamics? ¿ Where does the receptor bind to the capsid and what are the determinants that dictate species specificity?

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 MNV 是第一个鼠杯状病毒结构和中和研究。 人类杯状病毒是成人流行性胃肠炎的主要原因，因此 造成重大的发病率和经济损失。 对于人体健康来说并不重要 知道是否可以有效地针对这些病原体进行疫苗接种，并且 疫苗接种机制尚不明确。 因此，缺乏具有以下特征的小动物模型： 用于分析发病机制和免疫的广泛遗传和免疫学工具 阻碍了对这些重要病毒的研究。 H.W.维珍实验室有 发现了第一个鼠杯状病毒，他们将其命名为小鼠诺如病毒 1 或 MNV-1。 它们在杯状病毒中是独一无二的，它们已经发育出完整的动物和 细胞培养系统。 这为杯状病毒的基础研究提供了新的机遇 病毒学和免疫学。 具体来说，我们可以通过分析各种结构来解决一些问题 MNV-1： ¿ 抗体识别的重要结构决定因素是什么 给予保护？ ¿ 哪些类型的结构变化会导致病毒减毒？ ¿ 衣壳是动态的吗？中和/保护抗体通过影响这些来发挥作用吗？ 动力学？ ¿ 受体在哪里与衣壳结合以及决定的决定因素是什么 物种特异性？