SPHERICAL VIRUS STRUCTURES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 MNV是第一个小鼠钙化病毒结构和中和研究。 人类杯状病毒是成人流行性胃肠炎的主要原因， 造成严重的发病率和经济损失。 对人体健康很重要， 知道是否有可能有效地接种疫苗来对抗这些病原体，以及 疫苗接种机制尚未明确。 因此，缺乏小动物模型， 广泛的遗传学和免疫学工具，用于分析发病机制和免疫 阻碍了对这些重要病毒的研究 H.W.维珍实验室拥有 发现了第一个小鼠杯状病毒，他们命名为小鼠诺如病毒1或 MNV-1 在杯状病毒中是独一无二的，它们既能发育成一个完整的动物， 细胞培养系统 这为杯状病毒的基础研究提供了新的机会 病毒学和免疫学。 具体来说，我们可以通过分析各种结构来解决一些问题， MNV-1： 由抗体识别的重要结构决定因素是什么？ 给予保护？ 什么类型的结构变化导致病毒的减毒？ 衣壳是动态的吗？中和/保护抗体是否通过影响这些衣壳而起作用？ 动力学？ 受体在哪里与衣壳结合，决定其功能的决定因素是什么？ 物种特异性？

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. MNV is the first murine calcivirus structure and neutralization studies. Human caliciviruses are the major cause of epidemic gastroenteritis in adults, and thereby cause significant morbidity and economic loss. Important to human health, it is not known whether it is possible to effectively vaccinate against these agents, and mechanisms of vaccination are undefined. Thus, the lack of a small animal model with extensive genetic and immunologic tools for the analysis of pathogenesis and immunity has held back research on these important viruses. The H.W. Virgin laboratory has discovered the first murine calicivirus that they have designated Mouse Norovirus 1 or MNV-1. Unique amongst the Caliciviruses, they have developed both a whole animal and cell culture system. This affords new opportunities for basic research into calicivirus virology and immunology. Specifically, some of the questions we can address by analyzing the various structures of MNV-1: ¿ What are the important structural determinants that are recognized by antibodies that confer protection? ¿ What types of structural changes result in attenuation of the virus? ¿ Is the capsid dynamic and do neutralizing/protecting antibodies act by affecting these dynamics? ¿ Where does the receptor bind to the capsid and what are the determinants that dictate species specificity?