POTASSIUM CHANNEL SELECTIVITY FILTER

钾通道选择性过滤器

• 批准号: 8361639
• 负责人: Eduardo A Perozo
• 金额: $ 1.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361639
• 关键词: Event; Funding; Goals; Grant; Molecular; Molecular Analysis; Molecular Conformation; Mutation; National Center for Research Resources; Potassium Channel; Principal Investigator; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; design; mutant; prokaryotic potassium channel; sensor; structural biology; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The long-term goal of this project is to further understand the molecular mechanisms of gating in voltage-dependent channels, by focusing on the analysis of K+ channel gating in KcsA and KvAP. Analysis of the molecular identity of the pH sensor in the prokaryotic potassium channel KcsA identified several key mutants that stabilized the KcsA inner bundle gate in the fully open conformation at neutral pH, a fact that makes possible the crystallographic analyses of KcsA in a variety of functional states. Additional mutations in the selectivity filter were designed towards the determination of a fully conductive and an inactivated form of the channel and the probing of modal gating events.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本项目的长期目标是通过重点分析KcsA和KvAP中的K+通道门控，进一步了解电压依赖性通道门控的分子机制。在原核钾通道KcsA的pH传感器的分子身份的分析确定了几个关键的突变体，稳定KcsA内束门在完全开放的构象在中性pH值，一个事实，使得可能的KcsA在各种功能状态的晶体学分析。选择性过滤器中的额外突变被设计为确定通道的完全传导和失活形式以及探测模态门控事件。