MICRODIFFRACTION AT THE NE-CAT BEAMLINES

NE-CAT 光束线上的微衍射

• 批准号: 8361609
• 负责人: STEPHEN COPLAN HARRISON
• 金额: $ 2.33万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361609
• 关键词: Communities; Dimensions; Equipment; Felis catus; Funding; Goals; Grant; Methods; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Roentgen Rays; Sampling; Secure; Source; Structure; United States National Institutes of Health; beamline; cost; meetings; operation; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Due to increasing demand from our user community to be able carry out diffraction experiments with crystals smaller than 20-30 microns, during early 2007 the Resource implemented a microdiffraction facility at the 24-ID-E beamline. With that, we were able to deliver stable X-ray beams as small as 5 microns, which can be tailored to dimensions of the samples in order to reduce scattering contamination and backgrounds. This operating installation resulted in meeting one of our major Core 1 goals of providing the first such microdiffraction facility in the US. Also, this has resulted in some unique structures, which otherwise would not have been possible. The microdiffraction experiments at 24-ID-E beam-line were so successful and popular that our user community wanted us to replicate the exact same capability on our tunable beamline 24-ID-C as well. With the support of our core collaborators and HHMI investigators (S.C. Harrison, D. Eisenberg and E. Gouaux), we were able to secure funding (equipment grant to SCH) for a second MD2 for installation on 24-ID-C. During early 2009, 24-ID-C was outfitted with an MD2, similar to the one on 24-ID-E. While the first year of Core 1 research was spent on set-up, commissioning and user operations, during the second year we concentrated on developing methods for practical use of microdiffraction and enhancement of the microdiffraction capability.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 由于我们的用户对能够对小于20-30微米的晶体进行衍射实验的需求不断增加，2007年初，资源中心在24-ID-E光束线上安装了一个微衍射设备。有了这一点，我们能够提供小至5微米的稳定X射线光束，这种光束可以根据样品的尺寸进行定制，以减少散射污染和背景。这一操作安装实现了我们的主要核心1目标之一，即在美国提供第一个这样的微衍射设备。此外，这也导致了一些独特的结构，否则是不可能的。24-ID-E光束线上的微衍射实验是如此成功和受欢迎，以至于我们的用户社区希望我们也能在我们的可调谐光束线24-ID-C上复制完全相同的能力。在我们核心合作者和HHMI调查人员(S.C.Harison、D.Eisenberg和E.Gouaux)的支持下，我们能够获得资金(向SCH提供设备赠款)，用于在24-ID-C上安装第二个MD2。2009年初，24-ID-C配备了一个MD2，与24-ID-E上的类似。虽然第一年的核心研究花在设置、调试和用户操作上，但在第二年，我们集中精力开发微衍射的实际使用方法和增强微衍射能力。