STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS
STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS
批准号:
8361626
负责人:
AMY E KEATING
金额:
$0.16万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-04-01 至 2012-03-31
关键词:
Coiled-Coil DomainComputer AnalysisFundingGenetic TranscriptionGrantHumanMeasurementModelingNational Center for Research ResourcesPeptidesPrincipal InvestigatorProteinsProteomeResearchResearch InfrastructureResourcesSourceSpecificityStructureUnited States National Institutes of HealthYeastscombinatorialcostdesigndimerinterestprogramsspindle pole bodystructural biologytranscription factor
中文摘要
这个子项目是利用资源的许多研究子项目之一。
由NIH/NCRR资助的中心拨款提供。对子项目的主要支持
子项目的首席调查员可能是由其他来源提供的,
包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能
表示该子项目使用的中心基础设施的估计数量,
不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。
基廷实验室的研究旨在了解阿尔法螺旋螺旋线圈的结构特异性和相互作用特异性的决定因素,阿尔法螺旋螺旋线圈是蛋白质组中常见的相互作用基序。我们应用了一个集实验测量、计算分析和结构确定于一体的程序。特别感兴趣的靶标包括bZIP转录因子和酵母纺锤体极体的卷曲线圈。对于bZIP,调节转录的组合相互作用是通过盘绕线圈二聚体进行的。我们正在研究天然和合成的盘绕多肽,它们通过形成盘绕二聚体与人的bZIP相互作用。纺锤极体的蛋白质在预测的螺旋线圈结构域中高度丰富,我们正在对这些蛋白质进行表征,作为建立超大型纺锤杆体组件整体结构模型的一部分。
英文摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Research in the Keating lab aims to understand determinants of structural specificity and interaction specificity in alpha-helical coiled coils, a common interaction motif in the proteome. We apply an integrated program of experimental measurement, computational analysis, and structure determinantion. Targets of particular interest include bZIP transcription factors and coiled coils of the yeast spindle pole body. For the bZIPs, combinatorial interactions that regulate transcription are made via coiled-coil dimers. We are studying both native and synthetic coiled-coil peptides that interact with human bZIPs by forming coiled-coil dimers. Proteins of the spindle pole body are highly enriched in predicted coiled-coil domains, and we are characterizing these as part of an effort to build models of the overall structure of the very large spindle pole body assembly.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Computational and Experimental Investigation and Design of Protein Interaction Specificity
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批准号:10621973
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项目类别:
-
资助金额:$54.83万
-
财政年份:2023
-
负责人:AMY E KEATING
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依托单位:
Mapping, modeling and manipulating the interactions of protein domains that bind short linear motifs
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批准号:9575778
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项目类别:
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资助金额:$32.5万
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财政年份:2018
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负责人:AMY E KEATING
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依托单位:
Mapping, modeling and manipulating the interactions of protein domains that bind short linear motifs
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批准号:10242750
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项目类别:
-
资助金额:$32.67万
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财政年份:2018
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:9247955
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项目类别:
-
资助金额:$29.44万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:9039643
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项目类别:
-
资助金额:$29.44万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Analysis and design of protein interactions that regulate cell death
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批准号:10018034
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项目类别:
-
资助金额:$31.22万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:8849928
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项目类别:
-
资助金额:$29.44万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:8668226
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项目类别:
-
资助金额:$32.49万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
STRUCTURAL STUDIES OF INTERACTIONS AMONG BCL-2 FAMILY PROTEINS
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批准号:8361625
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项目类别:
-
资助金额:$0.16万
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财政年份:2011
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8328742
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项目类别:
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资助金额:$39.59万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Analysis and design of interaction specifically in proteins regulating apoptosis
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批准号:8054634
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项目类别:
-
资助金额:$6.07万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8538461
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项目类别:
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资助金额:$38.78万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Analysis and Design of Coiled Coil Partnering
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批准号:8138017
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项目类别:
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资助金额:$12.47万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8015704
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项目类别:
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资助金额:$41.19万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8527960
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项目类别:
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资助金额:$4.66万
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财政年份:2010
-
负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8722570
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项目类别:
-
资助金额:$39.47万
-
财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
STRUCTURAL STUDIES OF INTERACTIONS AMONG BCL-2 FAMILY PROTEINS
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批准号:8169242
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项目类别:
-
资助金额:$0.09万
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财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS
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批准号:8169243
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项目类别:
-
资助金额:$0.09万
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财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8149911
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项目类别:
-
资助金额:$37.59万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
STRUCTURAL SPECIFICITY OF MCL-1, A BCL-2 FAMILY PROTEIN
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批准号:7955134
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项目类别:
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资助金额:$0.25万
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财政年份:2009
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负责人:AMY E KEATING
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依托单位:
海外基金