STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS
STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS
批准号:
8361626
负责人:
AMY E KEATING
金额:
$0.16万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-04-01 至 2012-03-31
关键词:
Coiled-Coil DomainComputer AnalysisFundingGenetic TranscriptionGrantHumanMeasurementModelingNational Center for Research ResourcesPeptidesPrincipal InvestigatorProteinsProteomeResearchResearch InfrastructureResourcesSourceSpecificityStructureUnited States National Institutes of HealthYeastscombinatorialcostdesigndimerinterestprogramsspindle pole bodystructural biologytranscription factor
中文摘要
该子项目是利用资源的许多研究子项目之一
由NIH/NCRR资助的中心赠款提供。次级项目的主要支助
子项目的主要研究者可能是由其他来源提供的,
包括其他NIH来源。 为子项目列出的总成本可能
表示子项目使用的中心基础设施的估计数量,
NCRR赠款不直接向子项目或子项目工作人员提供资金。
Keating实验室的研究旨在了解α-螺旋卷曲螺旋中结构特异性和相互作用特异性的决定因素,α-螺旋卷曲螺旋是蛋白质组中常见的相互作用基序。我们采用实验测量、计算分析和结构测定的综合方案。特别感兴趣的靶标包括bZIP转录因子和酵母纺锤体极体的卷曲螺旋。对于bZIPs,调节转录的组合相互作用通过卷曲螺旋二聚体进行。我们正在研究天然和合成的卷曲螺旋肽,通过形成卷曲螺旋二聚体与人bZIPs相互作用。蛋白质的纺锤体极体高度富集在预测的卷曲螺旋结构域,我们正在表征这些作为一项努力的一部分,以建立模型的整体结构非常大的纺锤体极体大会。
英文摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Research in the Keating lab aims to understand determinants of structural specificity and interaction specificity in alpha-helical coiled coils, a common interaction motif in the proteome. We apply an integrated program of experimental measurement, computational analysis, and structure determinantion. Targets of particular interest include bZIP transcription factors and coiled coils of the yeast spindle pole body. For the bZIPs, combinatorial interactions that regulate transcription are made via coiled-coil dimers. We are studying both native and synthetic coiled-coil peptides that interact with human bZIPs by forming coiled-coil dimers. Proteins of the spindle pole body are highly enriched in predicted coiled-coil domains, and we are characterizing these as part of an effort to build models of the overall structure of the very large spindle pole body assembly.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Computational and Experimental Investigation and Design of Protein Interaction Specificity
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批准号:10621973
-
项目类别:
-
资助金额:$54.83万
-
财政年份:2023
-
负责人:AMY E KEATING
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依托单位:
Mapping, modeling and manipulating the interactions of protein domains that bind short linear motifs
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批准号:9575778
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项目类别:
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资助金额:$32.5万
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财政年份:2018
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负责人:AMY E KEATING
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依托单位:
Mapping, modeling and manipulating the interactions of protein domains that bind short linear motifs
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批准号:10242750
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项目类别:
-
资助金额:$32.67万
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财政年份:2018
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:9247955
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项目类别:
-
资助金额:$29.44万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:9039643
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项目类别:
-
资助金额:$29.44万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Analysis and design of protein interactions that regulate cell death
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批准号:10018034
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项目类别:
-
资助金额:$31.22万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:8849928
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项目类别:
-
资助金额:$29.44万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
Computationally guided design of helical peptide interaction reagents
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批准号:8668226
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项目类别:
-
资助金额:$32.49万
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财政年份:2014
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负责人:AMY E KEATING
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依托单位:
STRUCTURAL STUDIES OF INTERACTIONS AMONG BCL-2 FAMILY PROTEINS
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批准号:8361625
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项目类别:
-
资助金额:$0.16万
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财政年份:2011
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8328742
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项目类别:
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资助金额:$39.59万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Analysis and design of interaction specifically in proteins regulating apoptosis
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批准号:8054634
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项目类别:
-
资助金额:$6.07万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8538461
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项目类别:
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资助金额:$38.78万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Analysis and Design of Coiled Coil Partnering
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批准号:8138017
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项目类别:
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资助金额:$12.47万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8015704
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项目类别:
-
资助金额:$41.19万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
STRUCTURAL STUDIES OF INTERACTIONS AMONG BCL-2 FAMILY PROTEINS
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批准号:8169242
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项目类别:
-
资助金额:$0.09万
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财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS
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批准号:8169243
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项目类别:
-
资助金额:$0.09万
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财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
Very large datasets and new models to predict and design protein interactions
-
批准号:8722570
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项目类别:
-
资助金额:$39.47万
-
财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8527960
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项目类别:
-
资助金额:$4.66万
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财政年份:2010
-
负责人:AMY E KEATING
-
依托单位:
Very large datasets and new models to predict and design protein interactions
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批准号:8149911
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项目类别:
-
资助金额:$37.59万
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财政年份:2010
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负责人:AMY E KEATING
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依托单位:
STRUCTURAL SPECIFICITY OF MCL-1, A BCL-2 FAMILY PROTEIN
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批准号:7955134
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项目类别:
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资助金额:$0.25万
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财政年份:2009
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负责人:AMY E KEATING
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依托单位:
海外基金