UBIQUITIN C-TERMINAL HYDROLASE L3 COMPLEX WITH UBIQUITIN-BASED SUICIDE SUBSTRATE
UBIQUITIN C-TERMINAL HYDROLASE L3 COMPLEX WITH UBIQUITIN-BASED SUICIDE SUBSTRATE
批准号:
8361612
负责人:
Hidde L. Ploegh
金额:
$0.33万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-04-01 至 2012-03-31
关键词:
Antigen PresentationArchitectureBacteriaComplexDataFundingGrantHost Defense MechanismMalariaMembrane Protein TrafficMolecularNational Center for Research ResourcesOrthologous GeneParasitesPathway interactionsPlasmodium falciparumPrincipal InvestigatorProteinsRegulationResearchResearch InfrastructureResourcesSourceSpecificitySystemUbiquitinUnited States National Institutes of HealthVirusbasecostinterestmulticatalytic endopeptidase complexnovel strategiespathogenstructural biologysuicide substratesubiquitin C-terminal hydrolase
中文摘要
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英文摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
A new approach to controlling malaria-causing Plasmodium falciparum aims at the parasite's ubiquitin-proteasome pathway which is essential for its survival. Since the host's defense mechanisms against pathogens rely on the ubiquitin-proteasome system in antigen presentation and in the regulation of membrane trafficking, many viruses and bacteria encode proteins that act on the ubiquitin-proteasome pathway, yet there is a near-complete lack of data on this pathway in parasites.
It was shown that the mammalian UchL3 ortholog in Plasmodium falciparum (PfUchL3) possesses deubiquitinating as well as deNeddylating activity. We are interested in determining the molecular architecture of PfUchL3 and revealing the mechanisms for the dual specificity towards ubiquitin and NEDD8. To this end we prepared crystals of the complex between PfUchL3 and the ubiquitin based suicide substrate UbVME.
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Imposing order on the family of ubiquitin-conjugating (E2) enzymes through intracellular perturbation with nanobodies
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Sortase-mediated installation of recognition modules on T cells for redirected ki
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依托单位:
Endosomal TLRs and their accessory proteins: cell biology and biochemistry
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依托单位:
A new strategy to disrupt protein-protein interactions in eukaryotic cells.
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A new strategy to disrupt protein-protein interactions in eukaryotic cells.
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依托单位:
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依托单位:
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依托单位:
海外基金