STRUCTURAL STUDIES OF SAM RIBOSWITCHES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 核糖开关是一种结构化的RNA，它通过结合特定的小分子改变其构象，在转录或翻译水平上控制基因的表达。与俄亥俄州立大学的蒂娜·亨金斯博士合作，我们发表了两种类型的核糖开关的晶体结构，它们选择性地与S-腺苷蛋氨酸结合。这些结构为这些核糖开关中的配基识别机制提供了有价值的见解。我们试图利用APS束时间来确定SAM核糖开关在其载脂蛋白形式中的结构，并与不同的SAM类似物结合。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Riboswitches are structured RNAs that controls gene expression at either transcription or translation level by changing their conformation upon binding of specific small molecules. In collaboration with Dr. Tina Henkins at Ohio State University, we have published crystal structures of two types of riboswitches that selectively binds to S-adenosyl methionine (SAM). These structures provided valuable insights into the ligand-recognition mechanism in these riboswitches. We seek to use APS beam time to determine the structure of SAM riboswitches in their apo forms, and bound to different SAM analogs.