INTRA-CYTOPLASMIC COCCOLITH FORMATION IN COCCOLITHOPHORIDS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 球石生物是海洋单细胞藻类，以围绕每个细胞的错综复杂的方解石(CaCO3)结构(球石)而闻名。它们可以形成巨大的海洋水华，代表了地球上最大的钙化生物类别，并在许多方面构成了全球碳循环的基本部分。X射线断层扫描为研究上述因素提供了一种独特的方法。我们计划在二氧化碳浓度为300ppm和750ppm的情况下培养E.huxleyi。外层球虫将通过短暂暴露在温和的酸(pH 5.2)中溶解，并将细胞冷冻。我们将以适当的能量扫描以成像钙(L-边缘能量400 eV)、镁和碳(K边缘能量分别为1.31keV和0.28keV)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Coccolithophores are marine unicellular algae, notable for the intricate calcite (CaCO3) structures (coccoliths)which surround each cell. They can form vast oceanic blooms, representing the largest class of calcifying organisms on the planet and constituting a fundamental part of the global carbon cycle in a number of ways. X-ray tomography provides a unique way to investigate the factors described above. We plan to grow E. huxleyi at CO2 concentrations of 300 and 750 ppm.The outer coccoliths will be dissolved by brief exposure to mild acid (pH 5.2), and the cells frozen. We will scan at the appropriate energies to image calcium (L-edge energy 400 eV), magnesium and carbon (K-edge energies of 1.31 and 0.28 keV respectively).