95 GHZ 2D-ELDOR OF SPIN-LABELED PEPTIDES IN MEMBRANES
膜中自旋标记肽的 95 GHZ 2D-ELDOR
基本信息
- 批准号:8363964
- 负责人:
- 金额:$ 1.02万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-09-01 至 2012-08-31
- 项目状态:已结题
- 来源:
- 关键词:DiscriminationFrequenciesFundingGrantIon ChannelLipidsMembraneMembrane LipidsMembrane ProteinsMicrotome - medical deviceModelingMolecularNational Center for Research ResourcesPatternPeptidesPhysiologic pulsePrincipal InvestigatorPropertyRelaxationResearchResearch InfrastructureResolutionResourcesSamplingSourceSpin LabelsStructureTechniquesTechnologyTemperatureTimeUnited States National Institutes of Healthcholesterol analogcostgramicidin Ainsightinterestmagnetic field
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
2D ELDOR is a pulsed ESR technique that supplies greatly increased resolution to motional dynamics and local structure of biomolecules, such as membrane lipids and proteins, compared to conventional cw ESR techniques. In a recent study of the effect of the ion-channel forming peptide, gramicidin A, (GA) on model membrane structures, we could clearly distinguish and study the properties of both the boundary lipids that coat GA and the bulk lipids. That study at 17 GHz, however, indicated that higher frequency studies are needed, due to the increased orientational resolution they can provide. This enables better discrimination of the details of the ordering and dynamics. We have now extended the capabilities of our new high-power pulsed 95 GHz spectrometer to enable the study of spin-labeled lipids, such as the cholesterol analogue, CSL, and spin-labeled GA (GASL). The great challenges at 95 GHz are to have a sufficiently wide spectral coverage (as much as 350 MHz) to obtain the full nitroxide spin-label spectrum, and to have sufficiently short dead-times in view of the shortened T2 relaxation times at higher frequencies. We have succeeded, in particular, with macroscopically aligned membrane samples. Spectra have been obtained from GASL in aligned DPPC membranes just below room temperature. Using the new microtome technique, it was possible to obtain spectra for several orientations of the membrane normal with respect to the dc magnetic field. The parallel orientation shows the predominance of the molecular z- orientation, consistent with the way the GA aligns in DPPC. When the membrane is tilted perpendicular to the dc magnetic field, spectral contributions from the molecular x and y orientations become more pronounced as expected for such an aligned sample. What is of particular interest is that the 2D-ELDOR spectral patterns change with mixing time, Tm. This is a clear indication that these 2D-ELDOR spectra are showing slow-motional effects. That is, the various dynamic spin packet contributions to the spectrum are relaxing at different rates, leading to significant spectral changes with Tm. These results are expected to provide insight into the detailed dynamics of GA in the membranes.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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KEITH A EARLE其他文献
KEITH A EARLE的其他文献
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{{ truncateString('KEITH A EARLE', 18)}}的其他基金
INFORMATION ENTROPY METHODS AND EXPERIMENTAL DESIGN
信息熵方法与实验设计
- 批准号:
8364011 - 财政年份:2011
- 资助金额:
$ 1.02万 - 项目类别:
CONSTRUCTION OF NEW 170 & 250GHZ CW- ESR SPECTROMETER
新 170 的建设
- 批准号:
8363945 - 财政年份:2011
- 资助金额:
$ 1.02万 - 项目类别:
MULTIFREQUENCY ESR STUDIES OF PROTEIN DYNAMICS T4 LYSOZYME
T4 溶菌酶蛋白质动力学的多频 ESR 研究
- 批准号:
8363927 - 财政年份:2011
- 资助金额:
$ 1.02万 - 项目类别:
PORT OF NON LINEAR LEAST SQUARES & 1D SIMULATION SOFTWARE TO WINDOWS OS
非线性最小二乘法的端口
- 批准号:
8363930 - 财政年份:2011
- 资助金额:
$ 1.02万 - 项目类别:
PORT OF ESR SIMULATION & LEAST SQUARES FITTING SOFTWARE TO LINUX
ESR 模拟端口
- 批准号:
8363932 - 财政年份:2011
- 资助金额:
$ 1.02万 - 项目类别:
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