CONTROL SOFTWARE FOR STEP-FREQUENCY AND STEP FIELD EXPERIMENTS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在我们在95 GHz的2D-ELDOR取得初步成功之后，我们已经开发了执行ELDOR实验的步进频率版本所需的硬件，以研究动力学以及DEER，目标是研究自旋标记蛋白质中的距离。 我们现在正在开发适当的控制软件，以便将步进频率功能集成到我们现有的95 GHz脉冲光谱仪中。 该软件还将有一个钩子，使我们能够将步进场功能集成到我们可以在95 GHz执行的实验套件中。 这些灵活的脚本，基于公共领域的python脚本语言，将根据要求提供给我们满足服务请求的合作者和同事进行进一步开发。 我们希望这些脚本的知识和使用将有助于设计未来的实验，这将有助于我们解开复杂生物系统的结构和动力学的细节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. After our initial successes with 2D-ELDOR at 95 GHz, we have developed the hardware necessary for performing step-frequency versions of the ELDOR experiment, to study dynamics, as well as DEER, with the goal of studying distances in spin-labeled proteins. We are now working on the appropriate control software to integrate the step-frequency capability into our existing pulse spectrometer at 95 GHz. This software will also have a hook to enable us to integrate step-field capability into the suite of experiments we can perform at 95 GHz. These flexible scripts, based on the public domain python scripting language, will be made available on request for further development by collaborators and colleagues to whom we fulfill service requests. We expect that knowledge and use of these scripts will aid in the design of future experiments that will help us unravel the details of structure and dynamics in complex biological systems.