STOP CODON PROJECT

终止密码子计划

• 批准号: 8365828
• 负责人: PETER G SCHULTZ
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365828
• 关键词: Amber; Amino Acids; Biology; Escherichia coli; Funding; Fungal Genome; Genome; Grant; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; Terminator Codon; United States National Institutes of Health; cost; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Unnatural amino acids are incorporated through the suppression of the amber stop codon, as it is the less abundant stop codon in the genome of the E.coli. Thus it is likely to assume that the amber stop codon is supressed in different regions of the genome resulting in mutant proteins.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 非天然氨基酸通过琥珀终止密码子的抑制而掺入，因为它是大肠杆菌基因组中丰度较低的终止密码子。因此，可能假设琥珀终止密码子在基因组的不同区域被抑制，导致突变蛋白质。