STRUCTURE & DYNAMICS OF ELECTRON-TRANSFER INTERMEDIATES OF PHOTOSYNTHESIS

结构

• 批准号: 8364100
• 负责人: KLAUS MOEBIUS
• 金额: $ 0.08万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364100
• 关键词: Algae; Cyanobacterium; Electron Spin Resonance Spectroscopy; Electron Transport; Freezing; Frequencies; Funding; Grant; Methods; National Center for Research Resources; Organism; Photosynthesis; Photosystem I; Physiologic pulse; Preparation; Principal Investigator; Proteins; Proteobacteria; Reaction; Research; Research Infrastructure; Resources; Sampling; Site; Solutions; Source; Structure; System; Techniques; Technology; United States National Institutes of Health; Work; base; cost; design and construction; experience; instrumentation; mutant; novel; research study; technological innovation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Based on our previous high-field/high-frequency EPR work on protein systems , we will focus on orientation-resolved pulsed high-field EPR spectroscopy and related double-resonance techniques applied to wild type and site-specific mutants of photosynthetic organisms, in particular the reaction centers (RCs) from non-oxygenic purple bacteria and photosystems I and II (PS I, PS II) from oxygenic cyanobacteria and algae. The mutant RC preparations will be Fe-to-Zn substituted and studied by advanced multifrequency/multiresonance EPR techniques (operating at the MPI and at ACERT) in frozen-solution samples. The experimental methods used so far are planned to be extended by or combined with the unique instrumental methods available at ACERT, which are largely complementary to the EPR facilities at the MPI M¿lheim. This option, in conjunction with the immense experience in designing and constructing novel EPR instrumentation at ACERT, will result in innovations and technological advances beyond what is presently known in bio-oriented EPR spectroscopy. As an example, it is planned to combine the ACERT expertise in stopped-flow EPR in the 8-18 GHz range with our preliminary fast-flow EPR experiments at 95 GHz.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 基于我们之前对蛋白质系统的高场/高频 EPR 工作，我们将重点关注应用于光合生物野生型和位点特异性突变体的定向分辨脉冲高场 EPR 光谱和相关双共振技术，特别是来自非产氧紫色细菌的反应中心（RC）和光系统 I 和 II（PS I、PS II） 产氧蓝细菌和藻类。突变体 RC 制剂将被 Fe-to-Zn 取代，并通过先进的多频/多共振 EPR 技术（在 MPI 和 ACERT 上操作）在冷冻溶液样品中进行研究。迄今为止使用的实验方法计划通过 ACERT 提供的独特仪器方法进行扩展或与之结合，这些方法在很大程度上是对 MPI 米尔海姆的 EPR 设施的补充。这一选择与 ACERT 在设计和构建新型 EPR 仪器方面的丰富经验相结合，将带来超越目前已知的生物导向 EPR 光谱学的创新和技术进步。例如，计划将 ACERT 在 8-18 GHz 范围内的停流 EPR 方面的专业知识与我们在 95 GHz 范围内的初步快流 EPR 实验相结合。