STRUCTURE & DYNAMICS OF ELECTRON-TRANSFER INTERMEDIATES OF PHOTOSYNTHESIS

结构

• 批准号: 8364100
• 负责人: KLAUS MOEBIUS
• 金额: $ 0.08万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364100
• 关键词: Algae; Cyanobacterium; Electron Spin Resonance Spectroscopy; Electron Transport; Freezing; Frequencies; Funding; Grant; Methods; National Center for Research Resources; Organism; Photosynthesis; Photosystem I; Physiologic pulse; Preparation; Principal Investigator; Proteins; Proteobacteria; Reaction; Research; Research Infrastructure; Resources; Sampling; Site; Solutions; Source; Structure; System; Techniques; Technology; United States National Institutes of Health; Work; base; cost; design and construction; experience; instrumentation; mutant; novel; research study; technological innovation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Based on our previous high-field/high-frequency EPR work on protein systems , we will focus on orientation-resolved pulsed high-field EPR spectroscopy and related double-resonance techniques applied to wild type and site-specific mutants of photosynthetic organisms, in particular the reaction centers (RCs) from non-oxygenic purple bacteria and photosystems I and II (PS I, PS II) from oxygenic cyanobacteria and algae. The mutant RC preparations will be Fe-to-Zn substituted and studied by advanced multifrequency/multiresonance EPR techniques (operating at the MPI and at ACERT) in frozen-solution samples. The experimental methods used so far are planned to be extended by or combined with the unique instrumental methods available at ACERT, which are largely complementary to the EPR facilities at the MPI M¿lheim. This option, in conjunction with the immense experience in designing and constructing novel EPR instrumentation at ACERT, will result in innovations and technological advances beyond what is presently known in bio-oriented EPR spectroscopy. As an example, it is planned to combine the ACERT expertise in stopped-flow EPR in the 8-18 GHz range with our preliminary fast-flow EPR experiments at 95 GHz.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在我们以前高场/高频EPR工作的基础上，我们将重点介绍定向分辨脉冲高场EPR波谱和相关的双共振技术应用于光合作用生物的野生型和定点突变体，特别是来自非产氧紫色细菌的反应中心(RCS)以及来自含氧蓝细菌和藻类的光系统I和II(PS I，PS II)。突变的RC制剂将由铁取代为锌，并在冷冻溶液样品中用先进的多频/多共振EPR技术(在MPI和Acert操作)进行研究。到目前为止使用的实验方法计划通过ACERT提供的独特仪器方法进行扩展或与其结合，这些方法在很大程度上是对MPI M？lheim的EPR设施的补充。这一选择与ACERT在设计和建造新型EPR仪器方面的丰富经验相结合，将带来目前已知的生物导向EPR光谱学之外的创新和技术进步。作为一个例子，它计划将Acert在8-18 GHz范围内的停流EPR方面的专业知识与我们在95 GHz的初步快流EPR实验相结合。