QUANTITATIVE ANALYSIS OF AGE SPECIFIC VARIATION IN THE ABUNDANCE OF HUMAN FEMALE

人类女性丰度的年龄特异性变化的定量分析

• 批准号: 8365791
• 负责人: JAMES E MELVIN
• 金额: $ 0.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365791
• 关键词: Age; Biological Markers; Biology; Categories; Charge; Chromatography; Disease; Ductal; Female; Foundations; Funding; Fungal Genome; Future; Grant; Human; Immune response; Lactoferrin; Link; Monitor; Muramidase; National Center for Research Resources; Parotid Gland; Principal Investigator; Proteins; Proteomics; Reporting; Research; Research Infrastructure; Resources; Saliva; Salivary Proteins; Source; Technology; Testing; United States National Institutes of Health; Variant; Western Blotting; Woman; age group; age related; cost; defense response; histatin 1; human female; human old age (65+); normal aging; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of pooled saliva collected from the parotid glands of healthy female subjects, divided into two age groups 1 and 2 (20-30 and 55-65 years old, respectively). Hydrophobic charge interaction chromatography was used to separate high- from low-abundance proteins prior to characterization of the parotid saliva using multidimensional protein identification technology (MudPIT). Collectively, 532 proteins were identified in the two age groups. Of these proteins, 266 were identified exclusively in one age group, while 266 proteins were common to both groups. The majority of the proteins identified in the two age groups belonged to the defense and immune response category. Of note, several defense related proteins (e.g., lysozyme, lactoferrin and histatin-1) were significantly more abundant in group 2 as determined by G-test. Selected representative mass spectrometric findings were validated by Western blot analysis. Our study reports the first quantitative analysis of differentially regulated proteins in ductal saliva collected from young and older female subjects. This study supports the use of high-throughput proteomics as a robust discovery tool. Such results provide a foundation for future studies to identify specific salivary proteins which may be linked to age-related diseases specific to women.

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