STUDY OF THE DOPAMINE D1 AND D2 RECEPTORS LOCALIZATION IN VIVO

多巴胺 D1 和 D2 受体体内定位的研究

• 批准号: 8365781
• 负责人: EMILIANA BORELLI
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-20 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365781
• 关键词: Adult; Aftercare; Corpus striatum structure; DRD2 gene; Dopamine; Dopamine D1 Receptor; Dopamine D2 Receptor; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Goals; Grant; Laboratories; Mus; National Center for Research Resources; Neurons; Partner in relationship; Principal Investigator; Reporting; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; drug of abuse; in vivo; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The localization of dopamine D1 and D2 receptors is highly controversial. Some laboratories have reported that 100% of striatal neurons co-express the two receptors while others have reported that only a fraction of them does (from 5 to 20%. Our goal is to determine if both receptors co-localize in adult as well as in embryonal neurons. For this, we have generated in our lab knockin mice that express dopamine D1R tagged with ECFP and dopamine D2R tagged with EYFP. These mice will be used to visualize the localization of endogenous receptors under normal conditions and after treatment with drugs of abuse. Furthermore, we have mated D1R-ECFP with D2R-EYFP mice to be able to perform FRET studies that will tell us if these two receptors might interact in vivo.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 多巴胺D1和D2受体的定位是非常有争议的。一些实验室报道，100%的纹状体神经元共表达这两种受体，而另一些实验室报道，只有一小部分神经元共表达这两种受体（5 - 20%）。我们的目标是确定这两种受体是否在成人和胚胎神经元中共同定位。为此，我们在实验室中产生了表达用ECFP标记的多巴胺D1 R和用EYFP标记的多巴胺D2 R的敲入小鼠。这些小鼠将用于在正常条件下和滥用药物治疗后观察内源性受体的定位。此外，我们已经将D1 R-ECFP与D2 R-EYFP小鼠交配，以便能够进行FRET研究，从而告诉我们这两种受体是否可能在体内相互作用。