Control of Cytokine Gene Expression in LymphoidMyeloid Cells

我们正在分析淋巴样细胞中细胞因子和趋化因子基因表达的调控。我们选择干扰素- γ (ifn - γ)基因表达作为分析基因表达控制的模型系统。我们正在利用慢性ifn - γ表达的小鼠模型来确定对宿主的生物学后果以及这种表型与人类疾病的相关性。我们使用生物信息学方法来鉴定干扰素γ mRNA的3'非翻译部分的保守区域。我们认为这些保守区域代表了基因结构中重要的调控元件，除非mRNA的非编码区提供了一些进化优势，否则在进化过程中不会存在固有的保守区域。基于这一分析，我们针对小鼠干扰素- γ 3'非翻译区160 bp的区域进行了删除，因为该区域富含AUUA序列，并且该区域先前已被证明在细胞因子基因表达的调节中很重要。基因敲除（KO）小鼠已经成功创建，我们的数据表明，这种小鼠在用IL-12治疗后产生更多的干扰素- γ。此外，在基因敲除小鼠的血清中检测到低水平的干扰素- γ，而在野生型对照小鼠中则没有。此外，淋巴结、脾脏和胸腺的结构被破坏，肝脏表现出慢性炎症的迹象。随着CD4+和CD8+ T细胞的增加，T细胞的稳态被破坏，小鼠体内的T细胞具有更强的抑制活性。对抗原刺激，TH1反应增加，TH2反应减少。B细胞群也被改变，基线抗体产生是倾斜的。在胸腺中也观察到B细胞的频率增加，从而表明ifn - γ可能改变B细胞的运输。除了表型后果外，由于IgM和Ig2a抗体反应增加而IgG1反应减少，B细胞对抗原的反应也被破坏。强烈的抗dna和抗核抗原抗体反应也被观察到，这表明慢性ifn - γ表达可能在狼疮的发展中发挥作用。奇怪的是，在同样产生160 bp缺失的Balb/c中未见狼疮样症状。这些小鼠的脾脏增大，淋巴细胞转运到淋巴结有明显缺陷。这导致了一种与再生障碍性贫血非常相似的表型，我们正在阐明ifn - γ在这种疾病发展中的作用。此外，缺失的Balb/c小鼠似乎更能抵抗肾癌细胞系RENCA的攻击。综上所述，我们对干扰素- γ调控的多种机制的阐明表明了免疫效应细胞中干扰素- γ基因表达调控的复杂性。此外，我们现在已经开发了一个小鼠模型来理解和阐明长期慢性ifn - γ基因表达的系统生物学效应。

We are analyzing the regulation of cytokine and chemokine gene expression in lymphoid cells. We have chosen interferon-gamma (IFN-gamma) gene expression as a model system for analysis of the control of gene expression. We are utilizing mouse models of chronic IFN-gamma expression to determine the biological consequences to the host and rthe relevance of this phenotype to human disease.We have used a bioinformatics approach to identify conserved regions of the 3' untranslated portion of the interferon-gamma mRNA. It is believed that these conserved regions represent important regulatory elements in the gene structure as there would be no inherent region for conservation through evolution unless the non-coding regions of the mRNA provided some evolutionary advantage. Based on this analysis, we have targeted a 160-bp region of the murine interferon-gamma 3' untranslated region for deletion, as this region is rich in AUUA sequences and such regions have been previously shown to be important in the regulation of cytokine gene expression. The knockout (KO) mouse has been successfully created and our data indicates that this mouse produces significantly more interferon-gamma upon treatment with IL-12. In addition, low levels of interferon-gamma are detected in the serum of knockout mice but not wild type control mice. Furthermore, the architecture of lymph nodes, spleen and thymus is disrupted and the liver exhibits signs of chronic inflammation. T cell homeostasis has been disrupted as increased CD4+ and CD8+ T cells are present and the T reg cells in the mouse have more potent suppressor activity. There is also an increased TH1 response and a decreased TH2 response to antigenic stimulation. The B cell population is also altered and baseline antibody production is skewed. B cells are also observed in the thymus at increased frequency, thus indicating that IFN-gamma may alter B cell trafficking. In addition to the phenotypic consequences, the B cell response to antigen is also disrupted as increased IgM and Ig2a ab responses are seen with a decrease in the IgG1 response. Strong anti-DNA and anti-nuclear antigen antibody responses are also observed, suggesting that chronic IFN-gamma expression may play a role in the development of lupus. Curiously, lupus like symptoms are not seen in the Balb/c where the 160 bp deletion has also been generated. These mice have increased spleen sizes and an apparent defect in lymphocyte trafficking to the lymph nodes. This results in a phenotype very similar to aplastic anemia and we are elucidating the role of IFN-gamma in the development of this disease. In addition, the Balb/c mice with the deletion appear to be more resistant to challenge with the renal carcinoma line, RENCA. In summary, our approaches towards elucidating the multiple mechanisms involved in the regulation of interferon-gamma demonstrates the complexity by which interferon-gamma gene expression is regulated in immune effector cells. Furthermore we now have developed a mouse model for understanding and elucidating the systems biology effects of long term, chronic IFN-gamma gene expression.