Robust, defined, animal-free cell culture medium for the production of vaccines

描述(申请人提供)：Vero细胞系用于生产基于病毒的疫苗。基于Vero细胞系的细胞疫苗的使用正在增长。目前使用的大多数疫苗都是使用细胞培养基加血清生产的。在疫苗培养基中使用动物源性产品存在广泛传播传染病的风险。目前商业上可用的无血清疫苗培养基定义不明确，不一致，并且不能提供与补充血清的培养基相同的性能。我们建议生产一种性能优越的、明确的、无动物的、用于疫苗生产的细胞培养液。我们的项目回应了各机构对疫苗生产中动物来源成分的安全替代品的呼吁。 与公共卫生相关：Vero等上皮细胞系被用于生产基于病毒的疫苗，并且它们的用途正在扩大。目前商业上可用的无动物疫苗生产介质没有定义，也不能提供与添加血清的介质相同的性能。我们提出了业界首个明确的、高性能的无动物细胞培养液，以提高疫苗的安全性和产量。

DESCRIPTION (provided by applicant): The Vero cell line is used in the production of viral based vaccines. The use of cell based vaccines based on the Vero cell line is growing. The majority of the vaccines in use today are produced using cell culture medium supplemented with serum. The use of animal-derived products in vaccine culture media presents a risk for the transmission of infectious disease on a wide scale. Current commercially available serum-free vaccine media are not defined, inconsistent, and do not offer the same performance as media supplemented with serum. We propose to produce a superior performance, defined, animal-free, cell culture medium for vaccine production. Our project addresses the call by agencies for a safe alternative to animal-derived components in vaccine production. PUBLIC HEALTH RELEVANCE: Epithelial cell lines such as Vero are used in the manufacture of viral based vaccines and their use is expanding. Current commercially available animal-free vaccine production media are not defined and do not offer the same performance as media supplemented with serum. We propose the industry's first defined, high performance animal-free, cell culture medium to increase the safety and the production of vaccines.