Mentored Quantitative Research Career Development Award

指导定量研究职业发展奖

• 批准号: 8318098
• 负责人: Z. Hugh Fan
• 金额: $ 17.94万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-10 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8318098
• 关键词: Acute Lymphocytic Leukemia; Acute leukemia; Adult; Aftercare; American; Antibodies; Award; Base Sequence; Benchmarking; Binding; Biology; Biomedical Engineering; Biomedical Research; Blast Cell; Blood; Blood Cells; Blood Volume; Blood specimen; Bone Marrow; Cancer Biology; Cancer Relapse; Cause of Death; Cells; Cessation of life; Clinical; Compact discs; Cost Analysis; Detection; Detection of Minimal Residual Disease; Devices; Diagnosis; Diagnostic; Diagnostic Neoplasm Staging; Disease; Disease remission; Early treatment; Engineering; Ensure; Environment; European; Evaluation; Flow Cytometry; Genetic; Goals; Guidelines; Hematopoietic Stem Cell Transplantation; Immunophenotyping; K-Series Research Career Programs; Knowledge; Laboratories; Lead; Malignant - descriptor; Malignant Neoplasms; Medical; Medicine; Mentors; Methods; Microfluidics; Monitor; Nanotechnology; Nucleic acid sequencing; Outcome; Patients; Phenotype; Plastics; Polymerase Chain Reaction; Population; Prevention; Reagent; Reporting; Research; Residual Neoplasm; Residual state; Rotation; Sampling; Science; Screening for cancer; Solutions; Sorting - Cell Movement; Surface; Symptoms; Technology; United States; Validation; aptamer; base; cancer cell; cancer prevention; clinical care; design; experience; improved; innovation; leukemia; manufacturing process; milliliter; neoplastic cell; outcome forecast; peripheral blood; research and development; skills; treatment response

DESCRIPTION (provided by applicant): This "Mentored Quantitative Research Development Award" will facilitate the candidate, an engineer, to become a biologist. It will be achieved by taking courses to gain fundamental knowledge in biology/medicine and developing relevant research skills via mentored research. The selected courses will include core courses encompassing the fundamentals of biomedical sciences and advanced courses. Biomedical research experience will be obtained through lab rotations in the mentor's and co-mentor's laboratories. The mentored research is to develop a platform to concentrate and enrich acute leukemia cells for detection of minimal residual disease (MRD). MRD refers to the presence of residual malignant cells that remain in the patient during treatment or after treatment when the patient is in remission (no symptoms or signs of disease). The current benchmark method is not sufficiently sensitive for detecting all remaining tumor cells in peripheral blood. As a result, a platform with a higher sensitivity will be desirable since MRD can distinguish those who need intensive and potentially more toxic therapy from those who do not. The specific aims of this project are to (1) design and fabricate a device for enrichment of leukemia cells; (2) demonstrate the enrichment of leukemia cells in the device using aptamers with specific binding to acute lymphoblastic leukemia cells; and (3) validate the platform technology by analyzing leukemia cells in clinical samples and detect MRD. The significance of the research lies in the following aspects. First, the proposed research will lead to a platform for a rapid and efficient enrichment of acute leukemia cells. The platform will offer a measureable approach to determine if any additional chemo-therapy is needed; this will bring significant benefits to leukemia patients since unnecessary toxic therapy can be avoided. Second, the platform can be easily adapted for enrichment of rare circulating cells of other cancers. Isolation of these rare tumor cells could offer an opportunity for early-stage cancer screening and medical diagnostics. Third, the proposed research will advance the fields of cancer biology, biomedical engineering, and micro-nanotechnologies.

描述(申请者提供)：这一“导师定量研究发展奖”将帮助候选人，一名工程师，成为一名生物学家。它将通过选修课程获得生物学/医学的基本知识，并通过指导研究发展相关的研究技能来实现。入选的课程将包括生物医学基础的核心课程和高级课程。生物医学研究经验将通过在导师和合作导师的实验室中轮换获得。指导研究是开发一个浓缩和浓缩急性白血病细胞的平台，用于检测微小残留病(MRD)。MRD是指在治疗期间或治疗后，当患者处于缓解状态(没有疾病症状或体征)时，残留在患者体内的恶性细胞。目前的基准方法对于检测外周血中所有剩余的肿瘤细胞不够灵敏。因此，一个灵敏度更高的平台将是可取的，因为MRD可以区分那些需要强化治疗和可能更毒的治疗的人和那些不需要的人。本项目的具体目标是(1)设计和制造一种白血病细胞浓缩装置；(2)利用与急性淋巴细胞白血病细胞具有特异性结合的适配子展示在该装置中对白血病细胞的浓缩；(3)通过分析临床样本中的白血病细胞并检测MRD来验证平台技术。本研究的意义在于以下几个方面。首先，拟议的研究将为快速有效地浓缩急性白血病细胞提供一个平台。该平台将提供一种可测量的方法来确定是否需要任何额外的化疗；这将给白血病患者带来重大好处，因为可以避免不必要的毒性治疗。其次，该平台可以很容易地用于浓缩其他癌症的稀有循环细胞。分离这些罕见的肿瘤细胞可以为早期癌症筛查和医学诊断提供机会。第三，拟议的研究将推动癌症生物学、生物医学工程和微纳米技术领域的发展。