Microfluidics-enabled Immunoassay Array for Multianalyte Detection

用于多分析物检测的微流控免疫分析阵列

• 批准号: 8472502
• 负责人: Z. Hugh Fan
• 金额: $ 16.73万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-20 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8472502
• 关键词: Address; Aliquot; Antibodies; Antigen Targeting; Antigens; Biological; Biological Assay; Biological Markers; Biological Warfare; Biomedical Research; Chemicals; Cholera Toxin; Clinical; Consumption; DNA; Detection; Devices; Diagnostic; Diffuse; Diffusion; Dimensions; Disease; Elastomers; Elements; Escherichia coli O157; Food; Goals; Immunoassay; Individual; Infant; Laboratories; Lead; Liquid substance; Measures; Medical; Methods; Microfluidics; Modification; Monitor; Performance; Principal Investigator; Process; Production; Protein Microchips; Proteomics; Reaction; Reaction Time; Reagent; Research; Ricin; Salmonella typhimurium; Sampling; Site; Solid; Solutions; Surface; Techniques; Technology; Temperature; Terrorism; Testing; Time; Toxin; Traumatic Brain Injury; Variant; Water; antibody conjugate; base; cost; cost effective; design; drinking water; interest; nanoparticle; programs; prototype; safety testing; screening

DESCRIPTION (provided by applicant): The goal of the proposed research is to develop a platform technology for simultaneous detection of multiple analytes with better efficiency and lower cost. Currently, immunoassays are extensively used in biomedical research, clinical diagnostics, food/water safety testing, and biological warfare defense. Although they can be performed in a microplate (e.g. 96 wells) to increase sample throughput, the immunoassay in each well is generally limited to one analyte per test. Such a format is inefficient and not cost-effective for the requirement to detect multiple analytes in a sample. For instance, a spectrum of toxic agents should be simultaneously monitored in foods and drinking water. Similarly, a set of biomarkers, rather than an individual biomarker, should be detected for a particular disease to enhance the accuracy. Therefore, a platform to detect multiple analytes simultaneously is desirable for better efficiency and lower cost. The specific aims of the proposed research are to (1) design and optimize an array of microfluidic valves that are required for containing reagents, directing and regulating flows; (2) demonstrate the microfluidics-enabled multiplexed immunoassay array in the format of sandwich assays and study the effects of surface modification, flow rate, reagent concentration on the assay; and (3) validate the immunoassay array by detecting multiple analytes including toxins and biomarkers. The significance of the research lies in the following aspects. First, the proposed research will lead to a platform for simultaneous detection of multiple analytes. Such a capability will lead to efficient and cost- effective methods. Secondly, the microfluidic-enabled immunoassay array will have shorter analysis time and a reduction in the consumption of required sample and reagents. These advantages could have a significant impact since immunoassays are widely used in chemical, biological and clinical laboratories. Thirdly, the proposed research will result in a manufacturable process that allows mass production of low-cost, disposable devices. The ability to be disposable after a single use could have a tremendous impact to applications where cross-contamination of sequential samples is of concern.

描述（由申请人提供）：本研究的目标是开发一种平台技术，以更高的效率和更低的成本同时检测多种分析物。目前，免疫分析广泛应用于生物医学研究、临床诊断、食品/水安全检测和生物战防御。尽管可以在微孔板（例如 96 孔）中进行以增加样品通量，但每个孔中的免疫测定通常仅限于每次测试一种分析物。对于检测样品中的多种分析物的要求来说，这种形式效率低且不经济。例如，应同时监测食品和饮用水中的一系列有毒物质。同样，应该针对特定疾病检测一组生物标志物，而不是单个生物标志物，以提高准确性。因此，为了提高效率和降低成本，需要一个同时检测多种分析物的平台。本研究的具体目标是（1）设计和优化一系列微流体阀，这些阀用于容纳试剂、引导和调节流量； (2) 以夹心分析的形式展示微流控多重免疫分析阵列，并研究表面修饰、流速、试剂浓度对分析的影响； (3) 通过检测包括毒素和生物标志物在内的多种分析物来验证免疫分析阵列。研究的意义在于以下几个方面。首先，拟议的研究将建立一个同时检测多种分析物的平台。这种能力将带来高效且具有成本效益的方法。其次，微流控免疫分析阵列将缩短分析时间并减少所需样品和试剂的消耗。由于免疫测定广泛应用于化学、生物和临床实验室，这些优点可能会产生重大影响。第三，拟议的研究将产生一种可制造工艺，允许大规模生产低成本的一次性设备。单次使用后即可丢弃的能力可能会对需要关注连续样品交叉污染的应用产生巨大影响。

项目成果

The use of polyurethane as an elastomer in thermoplastic microfluidic devices and the study of its creep properties.

• DOI: 10.1002/elps.201300160
• 发表时间: 2014-02
• 期刊: ELECTROPHORESIS
• 影响因子: 2.9
• 作者: Gu, Pan;Nishida, Toshikazu;Fan, Z. Hugh
• 通讯作者: Fan, Z. Hugh