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Animal Models Core

动物模型核心

基本信息

批准号：
8379857
负责人：
G IAN GALLICANO
金额：
$ 16.59万
依托单位：
UNIVERSITY OF TX MD ANDERSON CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8379857
关键词：
Animal Model Animals Antineoplastic Agents Archives Biological Assay Breeding Cancer Patient Caring Cataloging Catalogs Cell Separation Cells Clinical Clinical Research Computer software Coupled Cryopreservation Data Databases Embryo Ensure Equipment Excision Fetus Fibroblasts Freezing Genetic Genotype Growth Health Housing Human Image Immunity Individual Injection of therapeutic agent Intestinal Cancer Knock-out Knockout Mice Liquid substance Location Longitudinal Studies Malignant Neoplasms Malignant neoplasm of gastrointestinal tract Malignant neoplasm of large intestine Medical Genetics Mission Modeling Molecular Mus Nitrogen Nude Mice Online Systems Operating Rooms Operative Surgical Procedures Outcome Pancreas Partner in relationship Patients Pharmaceutical Preparations Pharmacotherapy Predisposition Primitive foregut structure Printing Production Publishing Reaction Records Research Research Personnel Research Project Grants Resolution Role Running Sampling Schedule Services Site Small Intestines Sorting - Cell Movement Source Specific Pathogen Frees Stomach Testing Tissues Transgenic Mice Transgenic Model Transgenic Organisms Translational Research Tumor Tissue Ultrasonography Universities Work Xenograft procedure blastocyst chemotherapeutic agent cost efficacy testing embryonic stem cell germ free condition in vivo malignant stomach neoplasm member mouse model mutant neoplastic cell offspring programs pup sperm cell tissue preparation tumor

项目摘要

The Animal Models Core (AMC) will support all of the research projects in the program by providing the necessary genetically modified mice and producing low-passage in vivo human gastro-intestinal cancer explants in mice. One mission of the AMC will be to maintain mice for long-term studies to evaluate predisposition to cancer. A second mission of the AMC will be maintaining stocks of the genetically modified strains (e.g., elf, eif/Smad4, Smad4, Smad3, Cdk4-R24C mutant, cdk4 null mutant, htert knockout), and to breed these strains together to produce fetuses of double and triple mutant homozygous genotypes. Working with the Tissue Core B, these fetuses will be used as a source of embryonic fibroblasts and tumor cells by the projects. Pups with the desired double or triple mutant genotypes will constitute a large fraction of the progeny. To provide a continuous supply in support of the research projects a large amount of breeding, coupled with genotype analysis to identify the appropriate individuals will be required. A third mission of the AMC will be to provide synergy between the clinicians and projects testing anti-cancer drug therapies. This synergy will be in the form of producing, and providing husbandry of mice harboring genetic backgrounds predisposed to making tumors or nude mice xenografted with human tumor tissues for propagating lowpassage tumors. All mice will be produced and housed under barrier conditions that ensure continuing specific pathogen free status, Centralization of the production and care of these mice under the AMC will limit the number of individuals handling the animals, thereby further safeguarding their health. Moreover, by centralizing the production and dispensation of these genetically valuable animals, the animal core will be able to coordinate usage among the different projects, avoid wastage, and achieve an economy of scale that will permit these studies to be completed at less expense than if undertaken separately. A final mission of the AMC will be to serve as a synergistic hub for all projects and cores.

动物模型核心(AMC)将通过提供必要的转基因小鼠和体内低传代人胃肠癌的产生小鼠体内的外植体。AMC的任务之一将是维持小鼠进行长期研究，以评估易患癌症的人。AMC的第二项任务将是维持转基因食品的库存菌株(例如，ELF、eIF/Smad4、Smad4、Smad3、CDK4-R24C突变体、CDK4零突变体、hTERT基因敲除)和TO 将这些菌株混合在一起，产生双突变和三突变纯合子基因的胎儿。劳作有了组织核心B，这些胎儿将被用作胚胎成纤维细胞和肿瘤细胞的来源这些项目。具有所需的双重或三重突变基因的幼崽将构成很大一部分后代。为支持科研项目持续提供大量育种，再加上基因分析以确定合适的个体将是必要的。的第三个使命 AMC将在临床医生和测试抗癌药物疗法的项目之间提供协同作用。这协同的形式将是生产和饲养具有遗传背景的小鼠易于制作肿瘤或裸鼠移植人肿瘤组织用于低传代繁殖肿瘤。所有老鼠都将在屏障条件下生产和饲养，以确保持续在AMC下，这些小鼠的无特定病原体状态、集中生产和护理将限制接触动物的个人数量，从而进一步保障它们的健康。此外，通过集中生产和分配这些具有遗传价值的动物，动物的核心将是能够协调不同项目之间的使用，避免浪费，并实现规模经济将允许以比单独进行的费用更少的费用完成这些研究。的最后一项任务 AMC将成为所有项目和核心的协同枢纽。