Elucidation of molecular networks required to limit cardiac cell number

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During development, a variety of signals are coordinated that allow an organ to attain its proper size, which Is required for its normal function. In vertebrate heart development, several pathways are known to promote induction and growth of the heart, while few pathways are known to restrict the size of the heart. My long-term goal Is to understand how organs pnsperly achieve their size. The specific aims of this grant are to elucidate the molecular pathways and cellular mechanisms that are required to restrict the size of the heart during development using zebrafish. During the mentored phase of the award, I characterized a mechanism whereby hoxbSb, a downstream effector of RA signaling, non-autonomously restricts atrial cell number from the adjacent forelimb field. These studies helped to provide novel insights into organogenesis and potentially mechanisms which niay underly developmental syndromes affecting both the heart and the forelimb. Despite my initial studies, we still do not understand the nature of the increase in cardiac cells in RA signaling deficient embryos. In Specific Aim 1,1 will perform clonal analysis experiments to determine what cell fates may be directly affected by the increase In cardiac cells. Furthermore, we do not understand how downstream effectors of hoxbSb may be acting to restrict atrial cell number. In Specific Aim 2,1 will use loss of function approaches to characterize candidate effector genes downstream of hoxbSb that are required to specifically restrict atrial cell fonnatlon. From our initial screen of downstream effectors of RA signaling, we do not yet know of any genes that specifically restrict ventricular cell number. In Specific Aim 3,1 will use loss of function approaches to identify downstream effectors of RA signaling specifically required to limit ventricular cell number. It is likely other signaling pathways besides RA signaling are involved in restricting cardiac cell formation. In Specific Aim 4,1 will characterize novel mutants that can modify the Impact of RA signaling on heart size. Given the many contexts in which RA signaling acts, Including cardiac regeneration, lung branching, stem cell differentiation, and cancer biology, it is likely that my findings will also have a broad range of applications toward human health and the eventual development of therapeutics.