Claudin-7 in ovarian cancer

Claudin-7 在卵巢癌中的作用

• 批准号: 8552407
• 负责人: Patrice Morin
• 金额: $ 28.37万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8552407
• 关键词: Affect; Cancer cell line; Cell Line; Down-Regulation; Genes; Malignant neoplasm of ovary; Modeling; Oligonucleotide Microarrays; Pathway interactions; Pattern; Proteins; RNA Interference; Role; Signal Transduction; Technology; Tight Junctions; Tissues; Transfection; cancer cell; cell motility; claudin 3; claudin 4; migration; tumorigenesis

We have used a variety of approaches to characterize the patterns of claudin-7 expression in ovarian cancer. In addition, in order to identify the roles of claudin-7 in tumorigenesis, we have used RNAi technology to knockdown CLDN7 in two ovarian cancer cell lines (OVCA420 and OVCAR-2). Using this model, the effects of CLDN7 knockdown on proliferation, motility, and tight junction function have been studies. Illumina oligonucleotide arrays were then used to identify genes affected by CLDN7 down-regulation. We have identified several genes differentially expressed following CLDN7 knockdown. Interestingly, 200 genes were upregulated in both cell lines and 97 were downregulated in both cell lines. We are currently validating these genes and identifying pathways that may be important downstream of claudin-7. Once pathways are identified, we will use transfections and RNAi approaches to clarify the roles of these pathways in the transduction of claudin-7 signals. Interestingly, we have found that similar to claudin-3 and -4, claudin-7 can affect migration and invasion of ovarian cancer cells.

我们已经使用了多种方法来表征卵巢癌中claudin-7表达的模式。 此外，为了鉴定claudin-7在肿瘤发生中的作用，我们已经使用RNAi技术在两种卵巢癌细胞系（OVCA 420和OVCAR-2）中敲低CLDN 7。 使用该模型，研究了CLDN 7敲低对增殖、运动性和紧密连接功能的影响。 然后使用Illumina寡核苷酸阵列来鉴定受CLDN 7下调影响的基因。我们已经鉴定了CLDN 7敲低后差异表达的几个基因。有趣的是，200个基因在两种细胞系中上调，97个基因在两种细胞系中下调。 我们目前正在验证这些基因，并确定可能是claudin-7下游的重要途径。 一旦确定了通路，我们将使用转染和RNAi方法来阐明这些通路在claudin-7信号转导中的作用。有趣的是，我们发现与claudin-3和claudin-4相似，claudin-7可以影响卵巢癌细胞的迁移和侵袭。