Immunopathologic Studies

免疫病理学研究

• 批准号: 8432088
• 负责人: Robert B Colvin
• 金额: $ 13.31万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8432088
• 关键词: Acute; Adhesions; Allogenic; Allografting; Animals; Antibodies; Autopsy; Cells; Chronic; Complement Receptor; Dendritic Cells; Deposition; Doctor of Medicine; Electron Microscopy; Eragrostis; General Hospitals; Histology; Immunofluorescence Immunologic; Immunohistochemistry; Immunology; Immunoperoxidase Technics; In Situ; Inflammation; Injury; Instruction; Islets of Langerhans Transplantation; Kidney; Laboratory Research; Lesion; Liver; Location; Massachusetts; Molecular; Nature; Operative Surgical Procedures; Organ; Pathology; Pathway interactions; Process; Protocols documentation; Regulatory T-Lymphocyte; Research Personnel; Role; Sampling; Scanning; Slide; Techniques; Tissues; Toxic effect; Transplantation; Virus Diseases; capsule; cell type; complement C4d; cytokine; digital; embryonic stem cell; experience; immunopathology; intervention effect; islet; morphometry; professor; programs

Core A will perform all pathology tissue studies in this program project. The Core will be led by Robert B. Colvin, M.D., who is the Benjamin Castleman Distinguished Professor of Pathology at Harvard/Massachusetts General Hospital. He is an intenationally recognized investigator in transplantation pathology and has collaborated extensively in the past with the other investigators. He will be joined in this effort by R. Neal Smith, Associate Professor of Pathology, who has expertise in islet transplantafion pathology and Sandro Alessandrini, Assistant Professor of Surgery (Immunology), who has experience with endothelial activation pathways, Treg and dendritic cells. The studies will be performed in the Immunopathology Research Laboratory at the Massachusetts General Hospital (Thier 8). No embryonic stem cells will be used. The techniques that will be utilized include routine histology, immunohistochemistry, immunofluorescence, and electron microscopy. Immunoperoxidase techniques with a panel of mAbs will distinguish the infiltrating cell types adhesion and cytokine molecules and receptors, complement deposition (C4d), and endothelial activation markers (e.g., pERK). Analysis of the in situ processes will be enhanced by whole slide digital scans and morphometry. The pathology results will be correlated with functional and molecular studies done in the projects. In addition, complete necropsies will be done on all animals, with samples from all major organs analyzed by routine and special techniques as indicated.

核心A将执行本程序项目中的所有病理组织研究。核心将由罗伯特B领导科尔文，医学博士，他是哈佛/马萨诸塞州综合医院的本杰明·卡斯曼杰出病理学教授。他是移植病理学领域公认的研究者，过去曾与其他研究者广泛合作。他将加入这一努力的R。Neal Smith，病理学副教授，他在胰岛移植方面具有专长 病理学和Sandro Alessandrini，外科助理教授（免疫学），他有内皮激活途径，Treg和树突状细胞的经验。研究将在马萨诸塞州综合医院的免疫病理学研究实验室（Thier 8）进行。不会使用胚胎干细胞。 将使用的技术包括常规组织学、免疫组织化学、免疫荧光和电子显微镜。使用一组mAb的免疫过氧化物酶技术将区分浸润细胞类型粘附和细胞因子分子和受体、补体沉积（C4d）和内皮活化标志物（例如，pERK）。原位过程的分析将通过全载玻片数字扫描和形态测量学来增强。病理学结果将与项目中进行的功能和分子研究相关。此外，将对所有动物进行完整尸检，并根据指示通过常规和特殊技术分析所有主要器官的样本。