Innate Immune Gene Profiling Core

研究哮喘疾病的系统生物学方法中的一个关键组成部分是图谱。 转录组和免疫介体。在过去的十年中，我们小组在 利用包括来自商业来源的微阵列的各种cDNA图谱技术， 例如Affymetrix和Agilent，以及为实验室定制开发的技术，例如 基因的代表性差异分析、差异显示分析和序列分析 表情(SAGE)。最近，由于我们需要包括几个我们发现的新基因， 没有出现在任何商业阵列上，我们已经建立了自己的定制阵列 重点关注免疫和氧化反应中的重要基因。此外，我们还 调查了几个支持数据分析的基因网络途径程序。的主要目标是 这一核心是将我们的专业知识延伸到这个U19项目的调查人员，并提供一个用户友好的， 一站式设施，可广泛分析与免疫和免疫相关的基因和蛋白质阵列 氧化应激，这是这项提案中突出的两个问题。目标是：1.为了提供经过验证的、 为U19调查人员提供灵敏和标准化的人和鼠基因图谱技术。2. 在广泛的人和鼠细胞因子/趋化因子和免疫蛋白的使用方面提供帮助 数组。3.协助对来自cDNA和蛋白质阵列的数据进行生物信息学分析； CORE将在使用计算机程序分析数据方面提供访问和协助，以及 途径程序，以帮助解释数据，以了解其生物学意义。

A key component in the systems biology approach to the study of asthmatic disease is the profiling of transcriptome and immune mediators. Over the last decade, our group has published on the utilization of various cDNA profiling technology including microarrays from commercial sources, such as Affymetrix and Agilent, and technology developed custom to the lab, such as representational difference analysis, differential display analysis, and serial analysis of gene expression (SAGE). Recently due to our need to include several novel genes that we found which are not represented on any commercial arrays, we have established our own custom arrays focusing on genes important in immune and oxidative responses. In addition, we have also investigated several gene network- pathway programs to support data analysis. The main goal of this core is to extend our expertise to investigators in this U19 Project, and to provide a userfriendly, one-stop facility for the broad analysis of cDNA and protein arrays relevant to immunity and oxidative stress, two issues of prominence in this proposal. The goals are: 1. To provide validated, sensitive and standardized human and mouse cDNA profiling technology for U19 investigators. 2. To provide help in the usage of broad human and mouse cytokine/chemokine and immune protein arrays. 3. To assist in the bioinformatics analysis of data from the cDNA and protein arrays; the Core will provide access and assistance in using computer programs to analyze the data, and pathway programs to assist in the interpretation of data to understand its biological significance.