Broadband ultrasensitive detector for FTICR mass spectrometers

用于 FTICR 质谱仪的宽带超灵敏检测器

• 批准号: 8253082
• 负责人: ALEXANDER MISHARIN
• 金额: $ 16.69万
• 依托单位: MASSTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-10 至 2014-02-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8253082
• 关键词: Amplifiers; Biological; Biological Markers; Cells; Charge; Complex; Cyclotrons; Data; Detection; Device or Instrument Development; Devices; Disease; Electrodes; Electronics; Fourier Transform; Fourier transform ion cyclotron resonance; Goals; Home environment; Ions; Liquid substance; Magnetism; Mass Spectrum Analysis; Measurement; Measures; Metric; Motion; Noise; Ocular orbit; Peptides; Pharmaceutical Preparations; Phase; Post-Translational Protein Processing; Proteins; Proteome; Proteomics; Reporting; Resolution; Sampling; Semiconductors; Signal Transduction; Small Business Innovation Research Grant; Surveys; System; Testing; Time; Tissues; United States National Institutes of Health; base; cold temperature; comparative; cryogenics; design; detector; drug discovery; improved; instrument; magnetic field; mass spectrometer; metabolomics; novel; small molecule; superconducting quantum interference device

DESCRIPTION (provided by applicant): Increasing efficiency of ion detection in Fourier Transform Mass Spectrometry (FTMS) can have a tremendous impact on its applications in proteomics and entire analytical field. At present, the sensitivity of FTMS instruments is limited by electrical noise of semiconductor circuits utilized to amplify signals from electrodes of Ion Cyclotron Resonance cells. By using superconductor devices based on quantization of magnetic flux instead of semiconductor circuits we will improve sensitivity of the ion detection in FTMS by a factor of 10 or more. To reach high dynamic range in our desktop FTMS system, we also plan to design and test a new Ion Cyclotron Resonance cell, which will allow us to attain high mass resolution and mass accuracy with fewer analyzed ions. PUBLIC HEALTH RELEVANCE: Fourier Transform (FT) Mass Spectrometers (MS) are broadly used in proteomic and metabolomic studies of complex biological samples. These mass spectrometers demonstrate record-high mass accuracy and mass resolution that are vital to achieve high reliability in comparative analyses of normal vs disease proteomes. We plan to develop a novel detection system that will improve sensitivity and dynamic range of the FTMS systems by a factor of 10 or more, which will allow much better characterization of disease-specific biomarkers in the FTMS-based analysis of biological fluids or tissues.

描述（由申请人提供）：提高傅立叶变换质谱（FTMS）中离子检测的效率对其在蛋白质组学和整个分析领域的应用具有巨大影响。目前，FTMS仪器的灵敏度受到用于放大来自离子回旋共振池电极的信号的半导体电路的电噪声的限制。通过使用基于磁通量量子化的超导器件代替半导体电路，我们将使FTMS中离子检测的灵敏度提高10倍或更多。为了在我们的桌面FTMS系统中达到高动态范围，我们还计划设计和测试一种新的离子回旋共振池，这将使我们能够以更少的分析离子获得高质量分辨率和质量准确度。 公共卫生相关性：傅立叶变换（FT）质谱仪（MS）广泛用于复杂生物样品的蛋白质组学和代谢组学研究。这些质谱仪显示出创纪录的高质量准确度和质量分辨率，这对于在正常与疾病蛋白质组的比较分析中实现高可靠性至关重要。我们计划开发一种新的检测系统，将FTMS系统的灵敏度和动态范围提高10倍或更多，这将允许在基于FTMS的生物液体或组织分析中更好地表征疾病特异性生物标志物。