Molecular Virology and Systems Biology Screening Core

这个项目试图系统地解决关于先天与先天之间相互作用的关键问题 对HIV-1感染的反应。我们致力于剖析完整的细胞传感器和 效应器参与对HIV-1做出反应的先天信号通路。此外，限速措施 组件和这些路径之间的串扰将提供有关重要密钥的关键信息 在应对HIV-1病毒方面的参与者。我们也希望对HIV-1的运动学有一个新的理解 由这些途径调节的感染以及这些途径对感染的临床结果的影响。 对这些过程的系统级理解将被用来构建数学模型，这些模型可以 预测这些途径感染HIV-1的行为。这些集成的信息片段关于 宿主-病原体界面对于未来抗病毒和疫苗方法的优化将是非常有价值的。 在计划项目范围内进行的科学项目将在很大程度上依赖于大型 以高通量形式生产病毒和siRNA、shRNA和cDNA技术 这种方法的基因组学性质。分子病毒学与系统生物学筛选的目标 核心是支持科学家在各个方面的大规模和系统生物学筛选方法 通过提供最先进的细胞基因组学技术和分子病毒学工具。少年派有 在系统生物学领域有丰富的经验。科尼格博士将提供她在以下领域的集体专长 她有超过11年的病毒学经验，以及在开发和使用系统生物学筛查工具方面的经验。 这些研究有望提供对调节细胞和病毒过程的全球分子洞察力 对HIV感染的早期免疫反应。

This program project seeks to systemafically address crucial quesfions about the interplay between innate responses to HIV-1 infecfion. We anficipate to dissect the complete repertoire of cellular sensors and effectors involved in the innate signaling pathways that respond to HIV-1. Moreover, the rate-limifing components and the crosstalk between these pathways will provide critical information about important key players in the response to HIV-1. We also anficipate to acquire a novel understanding of the kinefics of HIV-1 infection regulated by these pathways and the effect of these pathways on the clinical outcome of infection. The systems-level understanding of these processes will be used to construct mathematical models that can predict the behavior of these pathways to HIV-1 infecfion. These integrated pieces of informafion about the host-pathogen interface will be invaluable for future opfimization of antiviral and vaccine approaches. Scientific projects taking place within the scope of the Program Project will rely to a great extent on largescale producfion of viruses and siRNA, shRNA and cDNA technologies in high-throughput format as a result of the genomics nature of the approach. The goal of the Molecular Virology and Systems Biology Screening Core is to support the scientists in all aspects ofthe large-scale and system-biology screening approaches by providing state-of-the-art cellular genomics technologies and molecular virology tools. The PI has significant experience in the field of systems-biology. Dr. KOnig will provide her collective expertise in Virology for over 11 years and her experience in developing and employing systems-biology screening tools. These studies are expected to provide global molecular insight into cellular and viral processes that regulate eariy immune responses to HIV infecfion.