Molecular Virology and Systems Biology Screening Core

分子病毒学和系统生物学筛选核心

• 批准号: 8013207
• 负责人: Renate Koenig
• 金额: $ 33.96万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8013207
• 关键词: Address; Animal Model; Antiviral Agents; Arts; Behavior; Biological Assay; Biological Models; Cell surface; Cells; Clinical; Cloning; Complementary DNA; DNA; Development; Equipment; Foundations; Future; Genes; Genomics; Goals; HIV; HIV Infections; HIV-1; Human Resources; Immune response; Infection; Instinct; Interferons; Kinetics; Mission; Molecular; Molecular Virology; Nature; Outcome; Pathway interactions; Process; Property; Reagent; Scientist; Screening procedure; Services; Signal Pathway; Small Interfering RNA; System; Systems Biology; Technology; Toll-like receptors; Transcript; Vaccines; Validation; Viral; Virus; base; cDNA Library; experience; follow-up; helicase; high throughput analysis; insight; large scale production; mathematical model; mutant; novel; novel therapeutics; particle; pathogen; programs; receptor; response; sensor; small hairpin RNA; therapeutic vaccine; tool; vector; virology

This program project seeks to systemafically address crucial quesfions about the interplay between innate responses to HIV-1 infecfion. We anficipate to dissect the complete repertoire of cellular sensors and effectors involved in the innate signaling pathways that respond to HIV-1. Moreover, the rate-limifing components and the crosstalk between these pathways will provide critical information about important key players in the response to HIV-1. We also anficipate to acquire a novel understanding of the kinefics of HIV-1 infection regulated by these pathways and the effect of these pathways on the clinical outcome of infection. The systems-level understanding of these processes will be used to construct mathematical models that can predict the behavior of these pathways to HIV-1 infecfion. These integrated pieces of informafion about the host-pathogen interface will be invaluable for future opfimization of antiviral and vaccine approaches. Scientific projects taking place within the scope of the Program Project will rely to a great extent on largescale producfion of viruses and siRNA, shRNA and cDNA technologies in high-throughput format as a result of the genomics nature of the approach. The goal of the Molecular Virology and Systems Biology Screening Core is to support the scientists in all aspects ofthe large-scale and system-biology screening approaches by providing state-of-the-art cellular genomics technologies and molecular virology tools. The PI has significant experience in the field of systems-biology. Dr. KOnig will provide her collective expertise in Virology for over 11 years and her experience in developing and employing systems-biology screening tools. These studies are expected to provide global molecular insight into cellular and viral processes that regulate eariy immune responses to HIV infecfion.

该计划项目旨在系统地解决有关先天之间相互作用的关键问题 对HIV-1的不合理的反应。我们对细胞传感器的完整曲目进行剖析和 对HIV-1响应的先天信号通路涉及的效应子。此外，限制速率 这些途径之间的组件和串扰将​​提供有关重要关键的关键信息 对HIV-1的回应玩家。我们还缺乏对HIV-1的动力的新了解 受这些途径调节的感染以及这些途径对感染临床结果的影响。 对这些过程的系统级别的理解将用于构建可以 预测这些HIV-1无限途径的行为。这些关于 宿主 - 病原体界面对于将来的抗病毒和疫苗方法的运输将是无价的。 在计划项目范围内进行的科学项目将在很大程度上依赖于LargesCale 因此，以高通量格式的病毒和siRNA，shRNA和cDNA技术的生产 该方法的基因组学性质。分子病毒学和系统生物学筛查的目标 核心是支持科学家在大规模和系统生物学筛选方法的各个方面 通过提供最先进的细胞基因组学技术和分子病毒学工具。 Pi具有 在系统生物学领域的重要经验。 Konig博士将提供她的集体专业知识 病毒学已有11多年的历史，以及她在开发和采用系统生物学筛查工具方面的经验。 预计这些研究将提供对调节细胞和病毒过程的全球分子见解 对艾滋病毒的无效反应。