Molecular Virology and Systems Biology Screening Core

该项目旨在系统地解决有关先天性 对HIV-1感染的反应。我们努力剖析细胞感受器的全部功能， 参与响应HIV-1的先天信号通路的效应物。此外，利率限制 组件和这些通路之间的串扰将提供关于重要关键的关键信息。 艾滋病病毒1型反应的参与者。我们也希望对HIV-1的动力学有一个新的认识 受这些途径调节的感染以及这些途径对感染临床结果的影响。 对这些过程的系统级理解将用于构建数学模型， 预测HIV-1感染途径的行为。这些整合的信息片段 宿主-病原体界面对于未来抗病毒和疫苗方法的优化将是非常宝贵的。 在计划项目范围内进行的科学项目将在很大程度上依赖于大规模的 病毒的生产和siRNA，shRNA和cDNA技术的高通量格式， 这种方法的基因组学性质。分子病毒学和系统生物学筛选的目标 核心是支持科学家在大规模和系统生物学筛选方法的各个方面 通过提供最先进的细胞基因组学技术和分子病毒学工具。主要研究者有 在系统生物学领域的丰富经验。KONIG博士将提供她的集体专业知识， 病毒学超过11年，她在开发和使用系统生物学筛选工具方面的经验。 这些研究有望为细胞和病毒过程提供全面的分子洞察力， HIV感染的早期免疫反应。

This program project seeks to systemafically address crucial quesfions about the interplay between innate responses to HIV-1 infecfion. We anficipate to dissect the complete repertoire of cellular sensors and effectors involved in the innate signaling pathways that respond to HIV-1. Moreover, the rate-limifing components and the crosstalk between these pathways will provide critical information about important key players in the response to HIV-1. We also anficipate to acquire a novel understanding of the kinefics of HIV-1 infection regulated by these pathways and the effect of these pathways on the clinical outcome of infection. The systems-level understanding of these processes will be used to construct mathematical models that can predict the behavior of these pathways to HIV-1 infecfion. These integrated pieces of informafion about the host-pathogen interface will be invaluable for future opfimization of antiviral and vaccine approaches. Scientific projects taking place within the scope of the Program Project will rely to a great extent on largescale producfion of viruses and siRNA, shRNA and cDNA technologies in high-throughput format as a result of the genomics nature of the approach. The goal of the Molecular Virology and Systems Biology Screening Core is to support the scientists in all aspects ofthe large-scale and system-biology screening approaches by providing state-of-the-art cellular genomics technologies and molecular virology tools. The PI has significant experience in the field of systems-biology. Dr. KOnig will provide her collective expertise in Virology for over 11 years and her experience in developing and employing systems-biology screening tools. These studies are expected to provide global molecular insight into cellular and viral processes that regulate eariy immune responses to HIV infecfion.