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Development and Application of Surface-Tethered Iterative Carbohydrate Synthesis

表面束缚碳水化合物迭代合成的开发与应用

基本信息

批准号：
8324617
负责人：
Keith J. Stine
金额：
$ 25.9万
依托单位：
UNIVERSITY OF MISSOURI-ST. LOUIS
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8324617
关键词：
Address Alzheimer&apos s Disease Area Attention Autoimmune Diseases Belief Biochemical Biological Biological Assay Biological Phenomena Biological Sciences Carbohydrate Chemistry Carbohydrates Cause of Death Chemicals Chemistry Collaborations Communication Communities Complex Development Diagnosis Diagnostic Disease Drug Delivery Systems Generations Genomics Glycobiology Glycoconjugates Glycolipids Goals Gold Grant Health Health Sciences Human Knowledge Laboratories Lead Learning Libraries Link Malignant Neoplasms Manuscripts Medical Methodology Methods Molecular Conformation Nanotechnology National Institute of General Medical Sciences Nature Oligosaccharides Organic Synthesis Participant Pathogenesis Pneumococcal Infections Polysaccharides Postdoctoral Fellow Preparation Prevention Process Property Proteomics Reaction Reporting Research Research Personnel Screening procedure Septicemia Solid Sorting - Cell Movement Stem cells Structure Students Surface Testing Therapeutic Therapeutic Agents Training Trust Work abstracting base carbohydrate binding protein combinatorial design experience flexibility glycosylation high throughput screening high throughput technology improved innovation interdisciplinary collaboration meetings method development monolayer multidisciplinary nanomaterials new technology novel novel strategies programs self assembly skills stem cell biology success sugar tool

项目摘要

Project Summary/Abstract Complex carbohydrates are involved in a broad variety of biological phenomena and their involvement in disease processes, in particular, have given this class of natural compounds a tremendous diagnostic and therapeutic potential. To this end, the PI's and co-PI's laboratories have focused their research efforts towards carbohydrates associated with diseases that rank among the top 10 leading causes of death worldwide, i.e. cancer, pneumococcal disease, septicemia, and Alzheimer's disease. At the core of this effort is the belief that if a comprehensive knowledge of the structure, conformation, and properties of these carbohydrates were available, elucidation of the mechanisms for the pathogenesis of these diseases could be facilitated. Consequently, this could lead to the development of effective tools for the prevention, diagnosis, and/or treatment of these diseases. The long-term goals of the PI's and co-PI's research programs are to make complex carbohydrates more accessible to general chemical, biochemical, medical and industrial audiences, and thereby keep pace with the exploding areas of glycobiology and related health sciences. Proposed herein is the expansion of studies initiated in our laboratories for the development of novel high-throughput technologies for oligosaccharide and glycoconjugate synthesis. At the core of this proposal is the development of a new strategy for supported synthesis called STICS (Surface-Tethered Iterative Carbohydrate Synthesis). The intellectual merit of the proposed research lies in its significant contributions to the field of basic and applied synthetic carbohydrate, surface, and nanomaterial chemistries upon its successful completion. Precisely, the success of this endeavor will not only advance the field of chemistries mentioned, but also fundamentally contribute to further development of glyco- and nanotechnology in general. We trust that the proposed developments will lead to the discovery of novel methodologies for the efficient synthesis and high throughput screening of carbohydrate-based libraries and therapeutics. A broader and long-term impact of this work relate to the professional training in surface, preparative organic, carbohydrate, bioorganic, and materials chemistry that undergraduate, graduate and post-doctoral research participants will receive. In addition, the multidisciplinary nature of the proposed research will allow students to expand their scientific base in an intellectual and practical sense. During their laboratory experience they will learn important tools that allow obtain, modify, and analyze organic molecules and nanomaterials by means of developing new methodologies and participating in the elaboration of state-of-the-art, multi-step syntheses. Student researchers will also become integrated into the scientific community through the preparation of manuscripts and attendance at professional meetings. As a consequence, students will develop better communication skills while also being exposed to scientific discourse. Finally, the proposed research will ultimately strengthen interdisciplinary collaborations with biomedical researchers.

项目总结/摘要复合碳水化合物参与了广泛的生物现象，它们参与了疾病过程，特别是，给这类天然化合物一个巨大的诊断和治疗潜力为此，PI和co-PI的实验室将其研究工作集中在碳水化合物与疾病有关，这些疾病是全球十大主要死亡原因之一，即癌症、肺炎球菌病、败血症和阿尔茨海默病。这一努力的核心是相信，如果对这些碳水化合物的结构、构象和性质的全面了解，如果能够获得这些药物，则有助于阐明这些疾病的发病机制。因此，这可能会导致开发有效的工具，用于预防，诊断和/或这些疾病的治疗。PI和co-PI研究计划的长期目标是使复杂的碳水化合物更容易获得一般化学，生物化学，医学和工业观众，从而跟上糖生物学和相关健康科学的爆炸性领域。本文中提出是我们实验室为开发新型高通量药物而开展的研究的扩展，低聚糖和糖缀合物合成技术。该提案的核心是发展一种新的支持合成策略，称为STICS（表面束缚迭代碳水化合物合成）。拟议研究的智力价值在于其对基础和成功完成后，应用合成碳水化合物、表面和纳米材料化学。准确地说，这一奋进的成功不仅将推动上述化学领域的发展，从根本上促进糖和纳米技术的进一步发展。我们相信提出的发展将导致发现新的方法，有效的合成和高基于碳水化合物的文库和治疗剂的通量筛选。这项工作的更广泛和长期的影响涉及表面，制备有机，碳水化合物，生物有机，材料化学，本科，研究生和博士后研究参与者将获得。此外，拟议研究的多学科性质将允许学生在知识和实践意义上扩大他们的科学基础。在他们的实验室经验，他们将学习重要工具，允许获得，修改，和分析有机分子和纳米材料，通过开发新的方法并参与最先进的多步合成的制定。学生研究人员也将成为融入科学界通过编写手稿和出席专业会议。因此，学生将得到更好的发展沟通技巧，同时也接触到科学话语。最后，拟议的研究将最终加强与生物医学研究人员的跨学科合作。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Square-wave voltammetry assays for glycoproteins on nanoporous gold.

DOI：
10.1016/j.jelechem.2014.01.009
发表时间：
2014-03-15
期刊：
Journal of electroanalytical chemistry (Lausanne, Switzerland)
影响因子：
0
作者：
Pandey B;Bhattarai JK;Pornsuriyasak P;Fujikawa K;Catania R;Demchenko AV;Stine KJ
通讯作者：
Stine KJ

Nanoporous gold as a solid support for protein immobilization and development of an electrochemical immunoassay for prostate specific antigen and carcinoembryonic antigen.