Co-axial Microinjection System for Freezing and Biopsy of Early Embryos

用于早期胚胎冷冻和活检的同轴显微注射系统

• 批准号: 8251450
• 负责人: Paul Jon Taylor
• 金额: $ 32.74万
• 依托单位: GENESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-05 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8251450
• 关键词: Animal Model; Animals; Area; Aspirate substance; Benchmarking; Biopsy; Businesses; Caliber; Cells; Collaborations; Communication; Communities; Contracts; DNA analysis; Development; Device Designs; Devices; Embryo; Embryo Research; Equipment; Evaluation; Evaluation Reports; Feedback; Fertility; Freezing; Glass; Goals; Hand; Herpes zoster disease; Human; Injection of therapeutic agent; Institutional Review Boards; Laboratories; Letters; Life; Liquid substance; Live Birth; Llama; Mails; Microinjections; Micromanipulation; Modeling; Modification; Movement; Mus; Penetration; Phase; Plastics; Procedures; Process; Production; Puncture procedure; Reporting; Research; Research Personnel; Research Project Grants; Side; Solutions; Staging; Structure; Suggestion; Surface; System; Technology; Telephone; Testing; Time; Uterus; Work; Writing; Zona Pellucida; base; blastocyst; blastomere structure; design; engineering design; experience; hatching; implantation; improved; instrumentation; interest; micromanipulator; prototype; research facility; research study; skills; sucking; tool; user-friendly

DESCRIPTION (provided by applicant): This Project, the Dracula Pipette Project, is intended to improve on a device invented by the Project Director (Dr. Paul Taylor) and to find ways to manufacture the device for general use. The device is a co-axial microinjection tool in which the injection pipette is presented from inside the holding pipette. This allows it to suck and hold a portion of the nearside of the surface of an embryo while an injection pipette is passed for injection, aspiration of fluid contents or biopsy of embryonic cells. The Dracula Pipette has been entirely handmade until now, and making it, especially making the delicate glass tips involved, has been too difficult and time-consuming to allow it to be used effectively by other researchers. The real significance of this device is that it allows control and manipulation of embryos at the hatched blastocyst stage of development, after hatching from the zona pellucida and before implantation in the uterus. After hatching, these embryos have no real structure and cannot be held on one side and penetrated from the opposite side, as is done routinely with embryos still in the zona. The human embryo exists for two to three days as a hatched blastocyst, and there has been no good tool for manipulating it at this most-differentiated stage before implantation. Because it is large and fluid-filled, the human hatched blastocyst is difficult to freeze successfully, and difficult to biopsy. Though the Dracula Pipette will surely be important for use with the hatched blastocysts of other species and on embryos at all stages before implantation, it is the only tool on the horizon for freezing and biopsy of human hatched blastocysts. The Small Business entity involved, GeneSearch, Inc., will supply the current best prototype of the device for testing and practical use by a variety of respected embryo researchers in a variety of laboratory settings, working with different kinds of animal models. The consultants will provide information about their experiences with the device and their ideas for improving or changing it for specific purposes. The inventor and the company Design Engineer will work to correct design problems as they are identified and will contract with various micro-technology firms for production of specific improved prototype parts. As these cycles of evaluation and upgrade continue, emphasis will shift to practical manufacture of all parts, so a robust, user- friendly device can be presented to the scientific community. PUBLIC HEALTH RELEVANCE: The improved Dracula Pipette, with disposable plastic tips and micromanipulator control, will be an important addition to the instrumentation in almost all biotech labs dealing with embryos. It will open areas of research in freezing and biopsy of large hatched blastocysts, areas previously ignored because the tool was lacking. Most importantly, it will give human fertility clinicians a way to freeze and biopsy human hatched blastocysts, embryos at the most differentiated stage of development before implantation in the uterus.

描述（由申请人提供）：该项目是Dracula移液器项目，旨在改进项目主管（Paul Taylor博士）发明的设备，并找到制造该设备供一般用途的方法。该设备是一种同轴微注射工具，其中从保持移液器内部显示注射移液器。这使其可以在传递注射移液器的同时吮吸并保持胚胎表面近距离的一部分，以进行注射，抽吸流体含量或胚胎细胞活检。直到现在，Dracula移液器一直是手工制作的，尤其是使其涉及精致的玻璃技巧，太困难且耗时，无法有效地被其他研究人员使用。该设备的真正意义在于，它允许在孵化的胚泡发育阶段，从Zona pellucida孵化和在子宫植入之前控制和操纵胚胎。孵化后，这些胚胎没有真实的结构，不能在一侧固定并从另一侧穿透，就像Zona中仍然存在的胚胎一样。作为孵化的胚泡，人类胚胎存在两到三天，并且在植入前最差异的阶段没有很好的工具来操纵它。由于它大且充满液体，因此人孵化的胚泡很难成功冻结，并且难以活检。尽管植入前的其他物种的孵化的胚泡和胚胎肯定对于与胚胎的孵化胚泡一起使用肯定很重要，但它是地平线上的唯一工具，用于冻结和活检人类孵化的胚泡。涉及的小型企业实体Genesearch，Inc。将为各种尊敬的胚胎研究人员在各种实验室环境中的测试和实际使用设备提供当前的最佳原型，并使用各种动物模型。顾问将提供有关他们在设备方面的经验以及为特定目的改进或更改其的想法的信息。发明家和公司设计工程师将在确定的设计问题上纠正设计问题，并将与各种微技术公司签约，以生产特定改进的原型零件。随着评估和升级的这些周期的继续，重点将转移到所有部分的实际制造中，因此可以向科学界呈现强大，用户友好的设备。 公共卫生相关性：带有一次性塑料尖端和微型手持控制的提高的Dracula移液器将是几乎所有处理胚胎的生物技术实验室中的仪器的重要补充。它将在冻结和活检的大型孵化胚泡的冻结和活检方面开放，因为缺乏该工具，因此以前被忽略的区域。最重要的是，它将为人类的生育临床医生提供一种冻结和活检人类孵化的胚泡的方法，在植入子宫之前，在最分化的发育阶段处于发育阶段。