Purchase of a Mass Directed Liquid Chromatograph

购买质量定向液相色谱仪

• 批准号: 8246818
• 负责人: Marisa C Kozlowski
• 金额: $ 37.16万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246818
• 关键词: Address; Alzheimer' s Disease; Area; Biochemistry; Biological; Catalysis; Chemicals; Chemistry; Detection; Excision; Fractionation; Funding; Generations; HIV; Ions; Libraries; Ligands; Liquid substance; Maintenance; Manuals; Methods; Organic Chemistry; Pennsylvania; Pharmacologic Substance; Process; Qualifying; Research; Research Personnel; Resources; Solvents; Stream; Synthesis Chemistry; System; Therapeutic Agents; Training; United States National Institutes of Health; Universities; anti-cancer therapeutic; experience; graduate student; instrument; instrumentation; novel; operation; programs

DESCRIPTION (provided by applicant): The proposed Mass Directed Liquid Chromatograph (MDLC) will address three key problems encountered in the execution of NIH funded programs at the University of Pennsylvania: (1) purification of compounds generated in a library format for biological study, (2) outlining the substrate scope of newly developed processes so that medicinal chemists find value in the methods, and (3) generation of novel ligands libraries to address unsolved problems in chemical catalysis. A limitation of the high throughput experimentation and library synthesis to address these problems is isolation of pure materials. For example, a typical manual purification of a single compound usually requires 2-6 h. A mass directed liquid chromatograph (MDLC) instrument interfaced to a glovebox train would automate the purification of even sensitive compounds (up to hundreds of compounds per day) by integrating UV-Vis and mass ion detection of product streams after passage through a separations column with automated fractionation and solvent removal. The proposed mass directed liquid chromatograph (MDLC) system will transform the capabilities of the groups utilizing organic synthetic chemistry at the University of Pennsylvania. Six highly active research groups in the area or organic methods, bioorganic, and biological chemistry comprised of approximately 85 undergraduate, graduate, and postdoctoral researchers have identified projects that would substantially benefit from this new resource and it is anticipated that at least six further groups will become major users within the first two years of operation. The instrumentation will accelerate NIH-funded research investigating basic synthetic organic chemistry for potential use in pharmaceutical synthesis, HIV inhibition, anticancer therapeutics, and agents for Alzheimer's treatment. Qualified staff within the Chemistry Department will oversee maintenance and training on this instrument, with substantial input from experienced users at Merck. Invaluable training will be afforded to graduate students and postdoctoral researchers that is absent outside of industrial settings.

描述（申请人提供）：拟议的质量导向液相色谱仪（MDLC）将解决在宾夕法尼亚大学执行NIH资助项目时遇到的三个关键问题：（1）纯化以文库形式产生的化合物用于生物学研究，（2）概述新开发的方法的底物范围，以便药物化学家在该方法中发现价值，以及（3）产生新的配体库以解决化学催化中未解决的问题。解决这些问题的高通量实验和文库合成的限制是纯材料的分离。例如，单一化合物的典型手动纯化通常需要2-6小时。与手套箱系列连接的质量导向液相色谱（MDLC）仪器将通过在通过具有自动分馏和溶剂去除的分离柱之后整合产物流的UV-Vis和质量离子检测来自动纯化甚至敏感的化合物（每天多达数百种化合物）。拟议的质量导向液相色谱（MDLC）系统将改变宾夕法尼亚大学利用有机合成化学的小组的能力。六个高度活跃的研究小组在该地区或有机方法，生物有机和生物化学组成的约85名本科生，研究生和博士后研究人员已经确定的项目，将大大受益于这一新的资源，预计至少有六个进一步的组将成为主要用户在头两年的操作。该仪器将加速NIH资助的研究，研究基础合成有机化学在药物合成，HIV抑制，抗癌治疗和阿尔茨海默氏症治疗药物中的潜在用途。化学部门的合格人员将监督该仪器的维护和培训，默克经验丰富的用户将提供大量意见。将为研究生和博士后研究人员提供工业环境之外缺乏的宝贵培训。