Purchase of a Mass Directed Liquid Chromatograph

描述(由申请人提供)：拟议的质量导向液相色谱仪(MDLC)将解决在宾夕法尼亚大学执行NIH资助项目时遇到的三个关键问题：(1)提纯以生物学研究的图书馆格式产生的化合物，(2)概述新开发的方法的底物范围，以便药物化学家在方法中找到价值，以及(3)生成新的配位体库，以解决化学催化中尚未解决的问题。解决这些问题的高通量实验和文库合成的一个限制是分离纯材料。例如，单一化合物的典型人工纯化通常需要2-6小时。连接手套盒系列的质量导向液相色谱仪(MDLC)仪器将在通过带有自动分馏和溶剂去除的分离柱后集成对产品流的UV-VIS和质量离子检测，从而自动净化甚至敏感的化合物(每天多达数百种化合物)。拟议的质量导向液相色谱仪(MDLC)系统将改变宾夕法尼亚大学利用有机合成化学的基团的能力。由大约85名本科生、研究生和博士后研究人员组成的有机方法、生物有机和生物化学领域的六个高度活跃的研究小组已经确定了将从这一新资源中大幅受益的项目，预计至少还有六个小组将在运营的头两年内成为主要用户。该仪器将加速NIH资助的研究，调查潜在用于药物合成、艾滋病毒抑制、抗癌疗法和阿尔茨海默氏症治疗药物的基础合成有机化学。化学系内合格的工作人员将监督该仪器的维护和培训，默克经验丰富的用户将提供大量意见。将为研究生和博士后研究人员提供宝贵的培训，这些培训在工业环境之外是不存在的。

DESCRIPTION (provided by applicant): The proposed Mass Directed Liquid Chromatograph (MDLC) will address three key problems encountered in the execution of NIH funded programs at the University of Pennsylvania: (1) purification of compounds generated in a library format for biological study, (2) outlining the substrate scope of newly developed processes so that medicinal chemists find value in the methods, and (3) generation of novel ligands libraries to address unsolved problems in chemical catalysis. A limitation of the high throughput experimentation and library synthesis to address these problems is isolation of pure materials. For example, a typical manual purification of a single compound usually requires 2-6 h. A mass directed liquid chromatograph (MDLC) instrument interfaced to a glovebox train would automate the purification of even sensitive compounds (up to hundreds of compounds per day) by integrating UV-Vis and mass ion detection of product streams after passage through a separations column with automated fractionation and solvent removal. The proposed mass directed liquid chromatograph (MDLC) system will transform the capabilities of the groups utilizing organic synthetic chemistry at the University of Pennsylvania. Six highly active research groups in the area or organic methods, bioorganic, and biological chemistry comprised of approximately 85 undergraduate, graduate, and postdoctoral researchers have identified projects that would substantially benefit from this new resource and it is anticipated that at least six further groups will become major users within the first two years of operation. The instrumentation will accelerate NIH-funded research investigating basic synthetic organic chemistry for potential use in pharmaceutical synthesis, HIV inhibition, anticancer therapeutics, and agents for Alzheimer's treatment. Qualified staff within the Chemistry Department will oversee maintenance and training on this instrument, with substantial input from experienced users at Merck. Invaluable training will be afforded to graduate students and postdoctoral researchers that is absent outside of industrial settings.