A Biacore T200 for Molecular Interaction Studies at the University of Miami

迈阿密大学用于分子相互作用研究的 Biacore T200

• 批准号: 8247257
• 负责人: RALF LANDGRAF
• 金额: $ 35.1万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247257
• 关键词: Antibodies; Applied Research; Basic Science; Binding; Clinical Research; Comprehensive Cancer Center; DNA; Detection; Equipment; Evaluation; Florida; Fostering; Funding; Future; Health; Housing; Human; Institutes; Label; Lead; Maintenance; Measurement; Medical; Methods; Modality; Molecular Weight; Nucleic Acids; Outsourcing; PTPRC gene; Pharmaceutical Preparations; Property; Proteins; Radial; Research; Research Personnel; Sampling; Serum; Structure; Surface Plasmon Resonance; Thermodynamics; United States National Institutes of Health; Universities; aptamer; falls; improved; instrument; instrumentation; medical schools; novel; protein complex; public health relevance; small molecule

DESCRIPTION (provided by applicant): The six basic research departments at the University of Miami, Miller School of Medicine carry out a broad range of basic science studies with strong translational components. Interdepartmental structures like the Comprehensive Cancer Center (which contributes to the maintenance of the proposed SPR equipment) foster interactions between basic researchers and with clinical research groups. Among this combined group of researchers (including one user from the neighboring Florida Scripps Institute), a strong need exists for modalities that allow highly sensitive and label free molecular interaction studies with small sample volumes. Surface Plasmon Resonance has become a major, if not the dominant, method of analysis for this type of applications in the last decade, especially with its increase in sensitivity in recent years (which now includes the label free binding studies of small molecule analytes) and the ability to multiplex and automate measurements. This allows for a broad range of studies to be carried out efficiently and with minimal sample volumes. Our high demand for SPR capability is in stark contrast to the absence of any such equipment with open access within a several hundred mile radius of UM. The absence of SPR capability forces researchers to rely on less sensitive and less informative methods at best. In some cases, such label free binding of novel, small molecule lead compounds, no other options are available. In yet other cases (antibody optimization) outsourcing is currently the only option The proposed purchase of a Biacore T200 would establish much needed SPR capability on the UM campus. The instrument will be housed in a supervised instrumentation facility will be maintained through institutional support. Featured applications include the characterization and optimization of therapeutically used antibodies, the evaluation of existing or de novo synthesized small molecular weight drugs, the detailed comparison of thermodynamic binding properties between ITC and SPR as alternative methods, the assembly of multi protein complexes on DNA, the characterization of SELEX derived nucleic acid aptamers to target proteins, and the detection of analyte concentrations in serum samples, and several projects emphasizing the strength of SPR in the study of weak interactions All applications fall within the capability range of a Biacore T200, and availability of SPR is certain to enhance progress on existing and future NIH-funded research. Public Health Relevance: Access to Surface Plasmon Resonance will accelerate existing and open up new research applications across the medical campus of UM. The impacted projects range from very basic to applied research with immediate translational potential. We therefore expect a highly synergistic impact on studies aimed at improving human health.

描述(由申请人提供)：迈阿密大学米勒医学院的六个基础研究部门开展范围广泛的基础科学研究，具有很强的翻译成分。像综合癌症中心这样的部门间结构(为拟议的SPR设备的维护做出贡献)促进了基础研究人员和临床研究小组之间的互动。在这组联合的研究人员中(包括一名来自邻近的佛罗里达州斯克里普斯研究所的用户)，存在着对允许小样本量的高灵敏度和无标记的分子相互作用研究的模式的强烈需求。在过去的十年里，表面等离子体共振已经成为这类应用的主要分析方法，特别是近年来其灵敏度的提高(现在包括小分子分析物的标记自由结合研究)以及多路复用和自动化测量的能力。这使得能够以最小的样本量有效地进行广泛的研究。我们对SPR能力的高需求与UM方圆几百英里范围内没有任何此类开放接入的设备形成鲜明对比。SPR能力的缺乏迫使研究人员充其量只能依赖不那么敏感和信息量较少的方法。在某些情况下，这种新型小分子先导化合物的无标记结合，没有其他选项可用。在其他情况下(抗体优化)，外包是目前唯一的选择 拟议购买Biacore T200将在密歇根大学园区建立亟需的SPR能力。仪器将存放在一个受监督的仪器设施中，仪器设施将通过机构支助进行维护。其特点的应用包括用于治疗的抗体的表征和优化，现有的或重新合成的小分子药物的评估，作为替代方法的ITC和SPR之间热力学结合性能的详细比较，多蛋白质复合体在DNA上的组装，SELEX衍生的核酸适配子对目标蛋白质的表征，以及血清样本中分析物浓度的检测，以及几个强调SPR在弱相互作用研究中的强度的项目，所有应用都在Biacore T200的能力范围内，SPR的可用性肯定会促进现有的和未来由NIH资助的研究的进展。 公共卫生相关性：获得表面等离子体共振将加速现有的研究应用，并在密歇根大学的整个医学园区开辟新的研究应用。受影响的项目范围从非常基础的研究到立即具有翻译潜力的应用研究。因此，我们期待对旨在改善人类健康的研究产生高度协同的影响。