Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
基本信息
- 批准号:8268442
- 负责人:
- 金额:$ 34.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-15 至 2015-05-31
- 项目状态:已结题
- 来源:
- 关键词:AffectAnimalsArchitectureBindingBiological AssayCellsChromatinComplexDepositionDevelopmentDiseaseDrosophila genusDrosophila inturned proteinEnvironmentEpigenetic ProcessFluorescenceGene Expression RegulationGenerationsGenesHematopoiesisImageImageryIn VitroIndividualInvestigationLabelLeadLifeMediatingMethodsModelingMolecularMultiprotein ComplexesNuclearPolycombProtein FragmentProteinsReadingRegulationResearch PersonnelResolutionSignal TransductionSpecificityTechnologyTestingTissuesTranscriptional RegulationTransgenic MiceUbiquitinValidationabstractinganimal tissuebasecell typecombinatorialdesignflexibilitygenetic regulatory proteininterestmeetingsmouse developmentprotein complexpublic health relevanceresearch study
项目摘要
DESCRIPTION (provided by applicant):
Abstract Visualization of the epigenetic states of cells in complex tissues is important since many cell types cannot be cultured in vitro and since the epigenetic states of cells can change during isolation and culture. Many tissues are composed of heterogeneous cell types and differences in epigenetic states among superficially identical cells affect their developmental and pathological potentials. We propose to develop technologies that enable visualization of the epigenetic states of individual cells in complex tissues of living animals. Epigenetic states consist of combinations of molecular marks in conjunction with multiprotein complexes that act on those marks. The consequences of different combinations of epigenetic marks are determined by combinatorial mechanisms. The functions of epigenetic regulatory proteins are determined by the combinations of interaction partners that are present in complexes formed by these proteins. We propose to develop technologies that enable visualization of combinatorial epigenetic marks, chromatin binding by epigenetic regulatory proteins, and epigenetic regulatory complexes formed by different combinations of interaction partners in animals. The technologies for visualization of combinatorial epigenetic marks and of epigenetic regulatory protein complexes in animals are to be based on fluorescent protein fragment complementation. Fragments of fluorescent proteins can associate with each other to produce a fluorescent complex when they are tethered in molecular proximity to each other. Technologies based on this principle are well suited for imaging epigenetic states in animals because (1) the direct fluorescence read-out is robust under challenging imaging conditions in animal tissues, (2) the high spatial resolution enables analysis of subnuclear localization, and (3) the lack of fluorescence of the individual fluorescent protein fragments produces a high signal to background ratio.
PUBLIC HEALTH RELEVANCE:
Project Narrative Animal cells use combinations of invisible tags to label genes that should be on or off in different kinds of cells growing in different environments. In this project, we develop molecules that enable researchers to see the combinations of tags in individual cells in living animals.
描述(由申请人提供):
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(4)
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TOM KLAUS KERPPOLA其他文献
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{{ truncateString('TOM KLAUS KERPPOLA', 18)}}的其他基金
Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
- 批准号:
8491945 - 财政年份:2010
- 资助金额:
$ 34.4万 - 项目类别:
Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
- 批准号:
8139012 - 财政年份:2010
- 资助金额:
$ 34.4万 - 项目类别:
Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
- 批准号:
8490698 - 财政年份:2010
- 资助金额:
$ 34.4万 - 项目类别:
Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
- 批准号:
8849059 - 财政年份:2010
- 资助金额:
$ 34.4万 - 项目类别:
Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
- 批准号:
9271347 - 财政年份:2010
- 资助金额:
$ 34.4万 - 项目类别:
Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using Fl
使用 Fl 观察动物组合表观遗传标记和复合物
- 批准号:
8663854 - 财政年份:2010
- 资助金额:
$ 34.4万 - 项目类别:
Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging F
新成像双分子荧光互补探针的优化
- 批准号:
7924978 - 财政年份:2009
- 资助金额:
$ 34.4万 - 项目类别:
Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging F
新成像双分子荧光互补探针的优化
- 批准号:
7556679 - 财政年份:2008
- 资助金额:
$ 34.4万 - 项目类别:
Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging F
新成像双分子荧光互补探针的优化
- 批准号:
8112653 - 财政年份:2008
- 资助金额:
$ 34.4万 - 项目类别:
Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging F
新成像双分子荧光互补探针的优化
- 批准号:
7694976 - 财政年份:2008
- 资助金额:
$ 34.4万 - 项目类别:
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