Animal Core

介绍 小鼠模型通常用于生物医学研究中作为临床前研究工具。例如，在开发用于治疗血友病A患者的新型药物时，血友病A小鼠模型通常用于证明概念的治疗证明以及研究比较性的治疗效果。 免疫原性血友病A小鼠模型，一种通过敲除外显子16产生，另一种通过敲除外显子17产生，首先在Haig Kazazian博士的实验室中开发（1，2）。迄今为止，已有超过400篇使用这些动物的出版物，并且商业开发或目前上市的几种生物制剂已在这些模型中进行了临床前试验。虽然有犬科动物 尽管生物医学研究界可获得血友病模型，但它们的数量极其有限，并且通常这些动物仅用于后期临床前试验。因此，目前拟议的项目仅涉及小鼠研究。目前不需要大型动物的支持。 在拟议的研究中，埃默里TRC-THD参与者可用于护理和维护小鼠的资源是大量的。埃默里大学在主校区内或附近的8座建筑物中设有动物设施，包括儿科ECC大楼，该提案的动物将被安置在那里并进行实验。另外的繁殖群也在白石大楼， 主校区。这两座建筑有60多个动物房，手术室，现代动物手术室，放射资源，诊断实验室和生物防护设施。有一个动物护理单位，确保日常维护和日常动物护理，包括提供适当的住房。 根据大学的政策提供卫生和营养。这项建议将受益于一个专门的核心啮齿动物饲养服务，由9名经理/主管和42名技术人员组成。此外，兽医服务单位提供整个动物保健计划。该计划的组成部分包括供应商监测、检疫和隔离、预防医学、日常观察、受伤或疾病的治疗和干预、哨兵动物的健康评估、尸检、组织病理学、寄生虫学、微生物学、血清学、血液学和血液化学。此外，实验室动物医学部门的工作人员可以提供动物麻醉、手术、X线摄影和 术后护理。它由两名教师DVM，两名居民DVM和六名兽医技术人员组成。 最后，埃默里大学致力于提供符合州和联邦法律以及公共卫生服务标准和政策的动物护理质量计划。该计划的标准符合或超过联邦动物福利法案PL 89-544（1966）及其后续修订案以及题为“实验动物护理和使用指南”和“公共卫生服务政策 实验动物的人道主义护理和使用”。自1992年以来，埃默里大学一直被国际实验动物护理评估和认证协会（AAALAC）认证。

Introduction Mouse models are routinely used in biomedical research as preclinical tools of investigation. For example, in the development of novel agents for the treatment of persons with hemophilia A, the hemophilia A mouse model is routinely used to demonstrate treatment proof of concept as well as investigate comparative immunogenicity. The hemophilia A mouse models, one generated by knockout of exon 16 and another by knockout of exon 17, were first developed in the laboratory of Dr. Haig Kazazian (1, 2). To date, there have been over 400 publications utilizing these animals and several biological agents in commercial development or currently marketed have been subjected to pre-clinical testing in these models. Although there are canine hemophilia models available to the biomedical research community, their numbers are extremely limited and typically these animals are only utilized for late stage preclinical testing. Therefore, the currently proposed projects only involve mouse studies. No support for large animals is requested at this time. Resources available to the Emory TRC-THD participants for the care and maintenance of mice in the proposed research are substantial. Emory University has animal facilities located in 8 buildings, on or near the main campus, including the Pediatric ECC building, where animals for this proposal will be housed and the experiments will be conducted. Additional breeding colonies are maintained in the Whitehead Building, also on the main campus. These two buildings have over 60 animal housing rooms, procedure rooms, modern animal surgeries, radiographic resources, diagnostic laboratories, and bio-containment facilities. There is an Animal Care Unit that ensures daily maintenance and routine animal care, including provision of proper housing. Sanitation and nourishment are provided based on the University's policy. This proposal will benefit from a specialized core rodent breeding services staffed by nine managers/supervisors and 42 technicians. In addition, a Veterinary Services Unit provides for the entire animal health care program. The components of this program include vendor surveillance, quarantine and isolation, preventive medicine, daily observation, treatment and intervention for injury or illness, health evaluations of sentinel animals, necropsy, histopathology, parasitology, microbiology, serology, hematology and blood chemistries. Additionally, the staff of the Laboratory Animal Medicine Unit can provide assistance with animal anesthesia, surgery, radiography and post-operative care. It is staffed by two faculty DVMs, two resident DVMs, and six veterinary technicians. Finally, Emory University is committed to providing a quality program of animal care in compliance with state and federal laws and the standards and polices of the Public Health Service. The standards of the program meet or exceed the federal Animal Welfare Act PL 89-544 (1966) and subsequent amendments and the documents entitled "Guide for the Care and Use of Laboratory Animals" and "Public Health Service Policy on Humane Care and Use of Laboratory Animals". Emory University has been continuously accredited campus wide by the Association for the Assessment and Accreditation of Laboratory Animal Care International (AAALAC) since 1992.