Animal Core

引言 小鼠模型通常用于生物医学研究，作为临床前研究的工具。例如，在开发用于治疗血友病A的人的新型药物时，血友病A小鼠模型通常用于证明治疗概念的证据以及研究比较 免疫原性。血友病A小鼠模型是由Hayg Kazazian(1，2)博士的实验室首次建立的，一种是由外显子16基因敲除产生的，另一种是由外显子17基因敲除产生的。到目前为止，已经有400多种出版物使用了这些动物，几种正在进行商业开发或目前上市的生物制剂已经在这些模型中进行了临床前测试。虽然有犬科动物 生物医学研究界可用的血友病模型，其数量极其有限，通常这些动物只用于晚期临床前试验。因此，目前提议的项目只涉及小鼠研究。目前不要求对大型动物提供支持。 在拟议的研究中，Emory TRC-THD参与者可用于照顾和维持小鼠的资源是大量的。埃默里大学在主校区内或附近的8栋建筑中设有动物设施，包括儿科ECC大楼，将在那里饲养本提案的动物并进行实验。在怀特黑德大楼里还保留着更多的繁殖群体， 主校区。这两座建筑有60多间动物收容室、程序室、现代动物外科手术、放射资源、诊断实验室和生物隔离设施。有一个动物护理股，确保日常维护和日常动物护理，包括提供适当的住房。 卫生和营养是根据大学的政策提供的。这项提议将受益于一个专门的核心啮齿动物饲养服务机构，该机构由9名管理人员/主管和42名技术人员组成。此外，兽医服务股还提供整个动物保健计划。该计划的组成部分包括供应商监测、检疫和隔离、预防医学、日常观察、受伤或疾病的治疗和干预、哨兵动物的健康评估、尸检、组织病理学、寄生虫学、微生物学、血清学、血液学和血液化学。此外，实验动物医学股的工作人员可以提供动物麻醉、外科手术、放射照相和 术后护理。它的工作人员包括两名教职员工DVM、两名常驻DVM和六名兽医技术人员。 最后，埃默里大学致力于提供符合州和联邦法律以及公共卫生服务标准和政策的高质量动物护理课程。该计划的标准达到或超过了联邦动物福利法PL 89-544(1966)和随后的修正案以及题为《保护和使用实验动物指南》和《公共卫生服务政策》的文件 埃默里大学自1992年以来一直获得国际实验动物评估和认可协会(AAALAC)的全校认证。

Introduction Mouse models are routinely used in biomedical research as preclinical tools of investigation. For example, in the development of novel agents for the treatment of persons with hemophilia A, the hemophilia A mouse model is routinely used to demonstrate treatment proof of concept as well as investigate comparative immunogenicity. The hemophilia A mouse models, one generated by knockout of exon 16 and another by knockout of exon 17, were first developed in the laboratory of Dr. Haig Kazazian (1, 2). To date, there have been over 400 publications utilizing these animals and several biological agents in commercial development or currently marketed have been subjected to pre-clinical testing in these models. Although there are canine hemophilia models available to the biomedical research community, their numbers are extremely limited and typically these animals are only utilized for late stage preclinical testing. Therefore, the currently proposed projects only involve mouse studies. No support for large animals is requested at this time. Resources available to the Emory TRC-THD participants for the care and maintenance of mice in the proposed research are substantial. Emory University has animal facilities located in 8 buildings, on or near the main campus, including the Pediatric ECC building, where animals for this proposal will be housed and the experiments will be conducted. Additional breeding colonies are maintained in the Whitehead Building, also on the main campus. These two buildings have over 60 animal housing rooms, procedure rooms, modern animal surgeries, radiographic resources, diagnostic laboratories, and bio-containment facilities. There is an Animal Care Unit that ensures daily maintenance and routine animal care, including provision of proper housing. Sanitation and nourishment are provided based on the University's policy. This proposal will benefit from a specialized core rodent breeding services staffed by nine managers/supervisors and 42 technicians. In addition, a Veterinary Services Unit provides for the entire animal health care program. The components of this program include vendor surveillance, quarantine and isolation, preventive medicine, daily observation, treatment and intervention for injury or illness, health evaluations of sentinel animals, necropsy, histopathology, parasitology, microbiology, serology, hematology and blood chemistries. Additionally, the staff of the Laboratory Animal Medicine Unit can provide assistance with animal anesthesia, surgery, radiography and post-operative care. It is staffed by two faculty DVMs, two resident DVMs, and six veterinary technicians. Finally, Emory University is committed to providing a quality program of animal care in compliance with state and federal laws and the standards and polices of the Public Health Service. The standards of the program meet or exceed the federal Animal Welfare Act PL 89-544 (1966) and subsequent amendments and the documents entitled "Guide for the Care and Use of Laboratory Animals" and "Public Health Service Policy on Humane Care and Use of Laboratory Animals". Emory University has been continuously accredited campus wide by the Association for the Assessment and Accreditation of Laboratory Animal Care International (AAALAC) since 1992.