Animal Core

动物核心

• 批准号: 8392592
• 负责人: John S. Lollar
• 金额: $ 31.54万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8392592
• 关键词: A Mouse; Accreditation; Amendment; Anesthesia procedures; Animal Housing; Animal Technicians; Animal Welfare Act; Animals; Autopsy; Biological; Biological Assay; Biological Products; Biomedical Research; Blood Chemical Analysis; Blood specimen; Breeding; Canis familiaris; Caring; Cell Line; Center for Translational Science Activities; Childhood; Clip; Commit; Communities; Containment; DNA; Data; Development; Diagnostic; Diagnostic radiologic examination; Disease; Ear; Ensure; Evaluation; Exons; Experimental Models; Faculty; Funding; Generations; Genotype; Health; Healthcare; Hematology; Hemophilia A; Hemorrhage; Hemostatic Agents; Hemostatic function; Histopathology; Housing; Human Resources; Individual; Injury; International; Intervention; Intravenous infusion procedures; Investigation; Knock-out; Laboratories; Laboratory Animal Medicine; Laboratory Animals; Laws; Maintenance; Marketing; Mesenteric Arteries; Microbiology; Modeling; Molecular; Mus; Operative Surgical Procedures; Parasitology; Participant; Persons; Plasma; Plasmids; Police; Policies; Postoperative Period; Preclinical Testing; Preventive Medicine; Procedures; Publications; Quarantine; RNA; Records; Research; Resources; Rodent; Sanitation; Sentinel; Serologic tests; Services; Specimen; Staging; Standardization; Tail; Thrombosis; Time; Tissue Harvesting; Tissues; Transgenic Mice; United States Public Health Service; Universities; Variant; Vendor; animal care; animal facility; artery occlusion; assay development; base; comparative; cyclophilin D; follow-up; guide for the care and use of laboratory animals; immunogenicity; inhibitor/antagonist; meetings; minimally invasive; mouse model; novel; pre-clinical; programs; research clinical testing; research study; tool; von Willebrand Disease

Introduction Mouse models are routinely used in biomedical research as preclinical tools of investigation. For example, in the development of novel agents for the treatment of persons with hemophilia A, the hemophilia A mouse model is routinely used to demonstrate treatment proof of concept as well as investigate comparative immunogenicity. The hemophilia A mouse models, one generated by knockout of exon 16 and another by knockout of exon 17, were first developed in the laboratory of Dr. Haig Kazazian (1, 2). To date, there have been over 400 publications utilizing these animals and several biological agents in commercial development or currently marketed have been subjected to pre-clinical testing in these models. Although there are canine hemophilia models available to the biomedical research community, their numbers are extremely limited and typically these animals are only utilized for late stage preclinical testing. Therefore, the currently proposed projects only involve mouse studies. No support for large animals is requested at this time. Resources available to the Emory TRC-THD participants for the care and maintenance of mice in the proposed research are substantial. Emory University has animal facilities located in 8 buildings, on or near the main campus, including the Pediatric ECC building, where animals for this proposal will be housed and the experiments will be conducted. Additional breeding colonies are maintained in the Whitehead Building, also on the main campus. These two buildings have over 60 animal housing rooms, procedure rooms, modern animal surgeries, radiographic resources, diagnostic laboratories, and bio-containment facilities. There is an Animal Care Unit that ensures daily maintenance and routine animal care, including provision of proper housing. Sanitation and nourishment are provided based on the University's policy. This proposal will benefit from a specialized core rodent breeding services staffed by nine managers/supervisors and 42 technicians. In addition, a Veterinary Services Unit provides for the entire animal health care program. The components of this program include vendor surveillance, quarantine and isolation, preventive medicine, daily observation, treatment and intervention for injury or illness, health evaluations of sentinel animals, necropsy, histopathology, parasitology, microbiology, serology, hematology and blood chemistries. Additionally, the staff of the Laboratory Animal Medicine Unit can provide assistance with animal anesthesia, surgery, radiography and post-operative care. It is staffed by two faculty DVMs, two resident DVMs, and six veterinary technicians. Finally, Emory University is committed to providing a quality program of animal care in compliance with state and federal laws and the standards and polices of the Public Health Service. The standards of the program meet or exceed the federal Animal Welfare Act PL 89-544 (1966) and subsequent amendments and the documents entitled "Guide for the Care and Use of Laboratory Animals" and "Public Health Service Policy on Humane Care and Use of Laboratory Animals". Emory University has been continuously accredited campus wide by the Association for the Assessment and Accreditation of Laboratory Animal Care International (AAALAC) since 1992.

介绍 小鼠模型通常在生物医学研究中用作临床前研究工具。例如，在开发用于治疗血友病患者的新型药物时，血友病A小鼠模型通常用于证明概念的治疗证明，并研究了比较 免疫原性。 Haig Kazazian博士（1，2）的实验室首次开发了由外显子16敲除外显子16产生的小鼠模型，另一种是由外显子17的敲除产生的。迄今为止，已经有400多个出版物利用这些动物，而在这些模型中，已经进行了商业开发或目前销售的几种生物制剂。虽然有犬 生物医学研究界可用的血友病模型非常有限，通常这些动物仅用于后期临床前测试。因此，当前提出的项目仅涉及小鼠研究。目前不要求大型动物的支持。 在拟议的研究中，埃默里（Emory）TRC-THD参与者可用于保养和维护小鼠的资源是大量的。埃默里大学（Emory University）在主要校园内或附近的8座建筑物中设有动物设施，包括儿科ECC大楼，该建筑将安置该提案的动物，并将进行实验。怀特海建筑物也维持了其他繁殖菌落 主校园。这两座建筑物设有60多个动物住房室，手术室，现代动物手术，射线照相资源，诊断实验室和生物接触设施。有一个动物护理部门可确保每日维护和常规动物护理，包括提供适当的住房。 根据大学的政策提供了卫生和营养。该建议将受益于由九名经理/主管和42位技术人员组成的专业核心啮齿动物育种服务。此外，兽医服务部门为整个动物卫生保健计划提供了。该计划的组成部分包括供应商的监视，隔离和隔离，预防医学，损伤或疾病的日常观察，治疗和干预，前哨动物的健康评估，死灵病，组织病理学，寄生虫学，微生物学，血清学，血液学，血液学和血液化学。此外，实验室动物医学部门的工作人员可以为动物麻醉，手术，射线照相和 术后护理。它由两名DVMS，两个居民DVM和六位兽医技术人员组成。 最后，埃默里大学（Emory University）致力于根据州和联邦法律以及公共卫生服务的标准和政策提供高质量的动物护理计划。该计划的标准符合或超过《联邦动物福利法》 PL 89-544（1966）以及随后的修正案以及题为“实验室动物的护理和使用指南”和“公共卫生服务政策的指南” 自1992年以来，埃默里大学（Emory University）的评估和认证协会（AAAALAC）对埃默里大学（Emory University）的校园不断认可。