Role of Beta-catenin in Epithelial Repair in Acute Lung Injury

描述（由申请人提供）：该基金旨在研究中性粒细胞在迁移过程中与上皮细胞相互作用激活肺上皮细胞2-连环蛋白依赖性信号通路的机制，以及这些通路在上皮修复中的重要性。我们将确定触发上皮细胞2-连环蛋白激活的信号通路，包括E-钙粘蛋白被PMN弹性蛋白酶裂解。我们将确定2- catenin激活促进体外PMN迁移诱导的上皮损伤修复的机制，并使用基因靶向小鼠建立ALI小鼠模型。

DESCRIPTION (provided by applicant): This grant seeks to investigate the mechanisms by which PMN interactions with epithelial cells during transmigration activate 2-catenin-dependent signaling pathways in lung epithelial cells and the importance of these pathways in epithelial repair. We will determine the signaling pathways that trigger epithelial 2-catenin activation including E-cadherin cleavage by PMN elastase. We will determine the mechanisms by which 2- catenin activation promotes repair of epithelial injury induced by PMN transmigration in vitro and in murine models of ALI using gene-targeted mice.