THE DROSOPHILA DORSAL AIR SACS, A MODEL SYSTEM FOR LUNG DEVELOPMENT

描述(申请人提供)：本研究的目的是了解果蝇背部气囊(DAS)的诱导、调节和模式发育和形态发生的过程。我们的工作表明，BNL-成纤维细胞生长因子诱导了这种大型、复杂和多叶的气管器官，其功能类似于脊椎动物的肺。这个器官为成年苍蝇通风，并为其飞行肌肉提供氧气。它的发展为研究导致器官诱导、生长和模式形成的途径提供了一个强大的系统。提出的具体目标是：(1)详细描述DAS的发育和形态；(2)鉴定和分析DAS中不同阶段和不同位置表达的基因；(3)鉴定和鉴定DAS发育中存在缺陷的突变体。

DESCRIPTION (provided by applicant): The goal of this research is to understand the processes that induce, regulate and pattern development and morphogenesis of the Drosophila dorsal air sacs (DAS). Our work has shown that Bnl-FGF induces this large, complex and multi-lobed tracheal organ which functions in ways that are analogous to the vertebrate lung. This organ ventilates the adult fly and supplies oxygen to its flight muscles. Its development offers a powerful system to study the pathways that lead to organ induction, growth and patterning. Specific aims are proposed to: (1) gain a detailed description of the development and morphology of the DAS; (2) identify and analyze genes expressed at various stages and at different locations in the DAS; and (3) identify and characterize mutants defective in DAS development.